HPLC

This paper describes the content of a well-written analytical procedure for regulated high-performance liquid chromatography (HPLC) testing. A stability-indicating HPLC assay for a drug product illustrates the required components for regulatory compliance, including additional parameters to expedite a laboratory analyst’s execution.

Scientists from around the world presented their groundbreaking technologies for in-field chromatography analysis during the International Symposium on Chromatography (ISC) in Liverpool, U.K.

Sechenov First Moscow State Medical University and Monash University Malaysia scientists recently tested HPTLC-guided preparative flash chromatography against olive flower extracts.

Here is some of the most popular content posted on LCGC International this week.

Posters at the 2024 conference will feature research on a variety of topics from PFAS testing to the HPLC analysis of soft drinks.

Waters Corporation and Princeton University scientists recently investigated how using elastic flow instability can improve the kinetic performance of chromatographic columns.

Here is some of the most popular content posted on LCGC International this week.

In this column, we look at the current version and the update of USP <621> on high-performance liquid chromatography (HPLC) that becomes effective 1st May 2025.

In a new study, scientists reflected on different advancements made in extracting, purifying, and quantifying kaempferol, a natural product present in various sources.

This “Column Watch” installment presents many of the highlighted topics and trends observed at the 52nd International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2024).

Scientists from Gansu Agricultural University recently used high-performance liquid chromatography (HPLC) to quantify 16 polyphenols in tomato fruits.

Scientists from the Federal University of Santa Catarina reviewed recent advancements made in the chromatographic analysis of endocannabinoids and phytocannabinoids in biological samples.

In this installment of “LC Troubleshooting,” we discuss strategies that can be used to minimize the likelihood of compound degradation in the isolation process.

In a new study, scientists developed a new high-performance liquid chromatography (HPLC) system to optimize miniaturized liquid chromatography (LC).

Northwestern Polytechnical University scientists recently tested a new approach for integrating micro-lenses and slits into portable liquid chromatography (LC) systems.

Dalian Maritime University scientists recently used high-performance liquid chromatography tandem triple quadrupole mass spectrometry HPLC-MS/MS to help detect amphetamine-type drugs in water.

Scientists from Università Politecnica delle Marche reported on baseline separation of isotopologoues of some amphetamine (AMP) derivatives on achiral and polysaccharide-based chiral columns.

During a presentation at the conference in Denver, Colorado scientists from Merck and Novilytic spoke about chromatography techniques used for different kinds of pharmaceutical analysis.

Scientists from Chemisches und Veterinäruntersuchungsamt Münsterland-Emscher-Lippe recently tested a new method for determining PFAS in food and food contact materials.

Keynote speakers at HPLC 2024 in Denver, Colorado will deliver talks on method development in liquid chromatography (LC), miniaturizing LC columns, and more.

This article explores three case studies where autosampler injection programs help to (i) reduce the carry-over observed for large nucleic acids during anion-exchange chromatography (AEX), (ii) reduce peak distortion and breakthrough for monoclonal antibodies (mAbs) during hydrophilic-interaction chromatography (HILIC), and (iii) facilitate dissolution of lipid nanoparticles (LNPs) so that a size-exclusion chromatography (SEC) method can be applied for ribonucleic acid (RNA) payload analysis.

In this edition of the LCGC Blog, Amber Hupp of the College of the Holy Cross provides advice for aspiring analytical chemists and shares accounts of other professionals.

In biotherapeutics manufacturing, controlling size variants of therapeutic monoclonal antibodies (mAbs) is critical for ensuring product quality.

Scientists from the University of Isfahan in Iran recently developed a new system for detecting and quantifying B vitamins in human samples.

This article discusses the challenges and effective solutions for high performance liquid chromatography (HPLC)-based analytical characterization of virus-like particles (VLPs).

In this month’s column, I highlight some of the primary considerations we face in method development and point to resources that can help users overcome uncertainty and develop highly effective 2D-LC methods.

In the world of liquid chromatography, innovative strides in column technology continue to take place. We are also reminded that there is always more to learn about “well-known” methodologies, and our craft is continuously influenced by important social concerns.

Governments are striving to implement policy changes towards a greater use of green technology, specifically around the generation of energy. Individuals and industrial organizations have also taken up the challenge, and now many companies are driving to significantly reduce their environmental footprint, or indeed become carbon negative within very short time frames.

An unexpected retention order for ketamine analogs was observed when using a biphenyl stationary phase for liquid chromatography-mass spectrometry (LC–MS).

In this edition of The LCGC Blog, Emanuela Gionfriddo discusses the two world-class scientists and trailblazer women in separation chemistry and the awards they received at Pittcon 2024.