Restek Corporation

· Traps and “delays” system-related PFAS, preventing interference · Allows accurate identification and quantitation of trace-level PFAS · Highly retentive-no breakthrough even with extended equilibration times

Method development for simultaneous analysis of ultrashort-chain, alternative, and legacy PFAS.

Method Development for Simultaneous Analysis of Ultrashort-Chain, Alternative, and Legacy PFAS

Testing shows that Restek Long-Life ESI electrodes for Sciex Turbo V sources provide equivalent chromatographic performance to original equipment manufacturer electrodes over significantly longer lifetimes.

Using a PFAS delay column is an effective way to separate target PFAS in samples from interferences caused by PFAS contamination in the LC system.

Using a PFAS delay column is an effective way to separate target PFAS in samples from interferences caused by PFAS contamination in the LC system.

This method showed excellent precision and accuracy for the 12 FDA regulated mycotoxins that were evaluated during a validation study that covered a variety of matrices.

This method showed excellent precision and accuracy for the 12 FDA regulated mycotoxins that were evaluated during a validation study that covered a variety of matrices.

By switching to a Raptor C18 column, labs can process more samples per hour while still meeting fluorochemical method requirements.

• Raptor C18 SPP 5 μm core-shell silica particle columns offer excellent resolution for fluorochemicals with short total cycle times. For even faster analysis, 2.7 μm core-shell particles are available. • Meets EPA Method 537 requirements. • Unique, robust Raptor C18 column design increases instrument uptime.

By switching to a Raptor C18 column, labs can process more samples per hour while still meeting fluorochemical method requirements.

Decrease analysis time for fluorochemicals with no sacrifice in chromatographic performance. Rapid perfluorinated alkyl analysis by LC-MS/MS increases sample throughput.

Decrease analysis time for fluorochemicals with no sacrifice in chromatographic performance. Rapid perfluorinated alkyl analysis by LC-MS/MS increases sample throughput.

Chromatographic conditions were developed for a fast GC-MS glycol ether analysis on the Rxi®-1301Sil MS column. This cyanobased thin film column provides better resolution and faster run times than the thick film cyanopropylphenyl-type columns commonly used for speciation of the glycol ethers. The glycol ethers are high production volume industrial chemicals that often occur as complex mixtures of isomers. The Rxi®-1301Sil MS column is uniquely matched for the separation of these isomers while still producing narrow and symmetric peaks for the low molecular weight ethylene glycol ethers.

Pain management LC analyses can be difficult to optimize due to the limited selectivity of C18 and phenyl-hexyl phases. In contrast, the selectivity of Raptor Biphenyl superficially porous particle (SPP) LC columns provides complete resolution of isobaric pain medications with a total cycle time of 5 min.

Semivolatile calibrations on this column dimension often range from 1.0 to over 100 ng/µL; however, a 0.25 mm ID column usually experiences peak overload as the mass on column approaches 10 ng. As shown in Figure 1, isobars that elute close together-such as benzo[b]fluoranthene and benzo[k]fluoranthene-quickly become unquantifiable as mass on column increases. Under split conditions, the resolution requirement (50% valley) is met for all nine calibration standards, and the peak apices shift less than 0.04 min, indicating only minor peak overload. Conversely, under splitless conditions, the three highest concentration calibration standards fail the resolution criterion. The peak fronting and resulting overlap from column overload make it impossible to generate a linear calibration including these points. Additionally, the peak apex of benzo[b]fluoranthene shifts more than 0.2 min, which could result in an erroneous compound identification.

Following sampling, tubes were transferred to a Markes UNITY™ thermal desorption system paired with an Agilent 7890B GC that was coupled to an Agilent 5977A MS detector. The UNITY™ system and GC–MS parameters are presented in Table I and Table II, respectively.