Application Notes

To overcome peak broadening, this application adjusts the column temperature for the bioZen WidePore C4 to improve peak shape in large molecule reversed-phase applications.

This application analyzes how to reduce peak widths by adjusting the flow rate for intact and reduced NIST mAb when using a bioZen WidePore C4 LC column.

Comparing core-shell superficially porous to fully porous particles to determine efficiency required for detection and separation of low-level impurities for subunit analysis.

Analyzing diverse method parameters, such as temperature, gradient program, and flow rates for optimization of intact mass applications when using bioZen WidePore C4.

Multiple C4 LC columns are compared to determine which provides the necessary separation of the impurity and avoids any spectral overlap in the MS detector.

bioZen WidePore C4 is built upon core-shell technology with bioinert hardware to offer optimal particle and pore-size morphology for high reproducibility and resolution.

Best practices with water in the analytical lab FAQs about lab water Lab water preparation optimized

To increase the resolution, this application evaluates and optimizes the gradient program when using a bioZen WidePore C4 LC column.

CE–MS can help address limitations associated with traditional LC–MS CE–MS provides fast separations with low sample consumption and no carry over

This technical overview describes the automated preparation of calibration solutions offered by the injector program available with Agilent autosamplers.

This technical overview describes the automated preparation of calibration solutions offered by the injector program available with Agilent autosamplers.

This application note presents a novel study of coffee aroma profiling by analyzing volatile organic compounds (VOCs) emitted at a series of progressively higher temperatures.

With Antec Scientific’s new ground-breaking µ-PrepCell-SS, electrochemical reduction of disulfide bonds in peptides, proteins, mAbs, and other biopharmaceuticals becomes possible routinely in LC-MS workflows. • Fast and efficient reduction of S-S bonds • Robust on-line reduction in top-down and bottom-up Proteomics • Reagent free, no interfering DTT or TCEP, ideal for HDXMS • Superior peptide sequencing and S-S bond assignment

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• Why hybrid systems are the wrong approach • The why, what, and how of CDS audit trail review • Understanding the Lifecycle Approach for Analytical Procedures

- Conventional light scattering comes with various limitations - New light scattering technology benefits protein/polymer characterization

In this study we demonstrate the benefits of the Thermo Scientific™ Orbitrap Exploris™ 240 LC–MS/MS for intact biopharmaceutical analysis under native conditions.

In this application note we evaluate the benefits of Real-Time Search for SPS MS3 (Orbitrap Eclipse MS) to enhance TMT quantification accuracy and proteome coverage.

In this application note we discuss the AcquireX workflow using the Orbitrap ID-X for detection and quantitation in non-targeted, multiresidue LC–MS pesticide profiling.

The Column spoke to Carlos Calderón about his research project, investigating pharmacolipidomics analysis of betulin in human primary keratinocytes to monitor alterations in the lipid profiles induced by treatment with betulin.

Incognito offers some tips for chromatographers during the current crisis