Application Notes

Particularly in the pharmaceutical industry, drug purity isn’t just a goal – it’s essential for achieving safety, stability and efficacy. However, purification is easier said than done, especially with challenging molecules like DNA and RNA “oligonucleotides,” due in large part to their diversity and the range of impurities that can be generated during production. Enter DAVISIL® chromatographic silica, with a wide range of pore diameters and particle sizes to meet your specific application, performance and sustainability requirements. Before you choose the chromatography resin for your next purification application, take a look at these 5 considerations.

Separation of free fatty acids from triglycerides can be inefficient and challenging, resulting in low yields. Whether using supercritical fluid chromatography or standard liquid chromatography, discover why DAVISIL® Chromatographic Silica has been referenced in third-party publications as the preferred separation medium because of its greater capacity. Learn more about Omega-3 Fatty Acids purification from Fish Oil in this technical data sheet.

Not all chromatography resins are created equal. Off-the-shelf chromatography resins might not always meet the rigorous purification requirements of biopharmaceutical manufacturing. Custom bonded silica from Grace can address a wide range of separation challenges, leading to real performance improvements. Discover more about the latest innovations in chromatography silica from Grace, including VYDAC® and DAVISIL®.

The optimized LC-MS/MS method covering 388 signaling lipids shows that the bioinert coated YMC Accura Triart C18 column combines high sensitivity with high resolution.

This application note describes the simultaneous extraction of aldosterone at low (< 25pg/mL) concentration and angiotensin I and II from plasma, with the potential to improve throughput and reduce time and costs in PRA determination.

App Note: This application notes demonstrates an automated Supported Liquid Extraction workflow in the analysis of Zolmitriptan, a selective serotonin receptor agonist, in plasma.

Customer Interview with Antonio Checa, a senior researcher at the Wheelock’s lab at the Karolina Institute describing automating the sample prep workflow in the analysis of lipid mediators such as oxylipinds.

Blog series: a break down of SPE Fundamentals to guide you in choosing the right tool and technique in solving bioanalytical problems.

Webinar: learn how Supported Liquid Extraction (SLE) can be an effective sample cleanup technique for Bioanalysis. The simple and efficient 3-step procedure provides consistent and reproducible results for a wide range of bioanalytical applications.

Webinar: Review of the fundamentals of common sample prep techniques, such as protein precipitation, liquid-liquid and supported liquid extraction, and solid-phase extraction, breaking down their mechanisms of action and highlighting strategies for choosing the best technique to use in bioanalysis.

Read about compliant analysis for the determination of ethanol and methanol content in fuels.

Discover an optimized GC/MS method conditions allowing faster analysis and increased throughput.

Read about this fast method providing excellent resolution and highly repeatable separations contributing to improve data quality while increasing lab productivity.

Discover how the PerkinElmer GC 2400™ Platform provides high level of precision for VOC analysis in paint.

Discover fast, robust, and sensitive analysis of alcohol in food with a new GC user-experience.

Discover an easy method that can help meet the most stringent purity requirements.

In this article, the results of a DIMS air monitoring campaign in Auckland, New Zealand, are described. Real-time SIFT–MS measurements were obtained at three sites over a period of several weeks. At each site, SIFT–MS provided effective on-site analysis to sub-ppbV levels with better than 1-min time resolution.

This application note describes the determination of method detection limits (MDLs) for the newly regulated HON (Hazardous Organic NESHAP) compounds, which validate SIFT-MS as an effective solution for measuring these toxic VOCs and other environmental pollutants in ambient air, whether at the fence line or in a mobile setting.

This study demonstrates high-throughput analysis of BTEX compounds from several matrices (air, water and soil). Detection limits in the single-digit part-per-billion concentration range (by volume) are readily achievable within seconds using SIFT-MS, because sample analysis is achieved without chromatography, pre-concentration, or drying. We also present a calibration approach that enables speciation of ethylbenzene from the xylenes in real-time.

This application note summarizes key SIFT-MS results presented in a peer reviewed article entitled “Unreported VOC Emissions from Road Transport Including from Electric Vehicles.” Learn how the Wolfson Atmospheric Chemistry Laboratory at the University of York used SIFT-MS for VOC analysis in its platform to experimentally verify that motor vehicle screen wash is a significant unreported source of VOC emissions (especially for ethanol and methanol).

This brochure SyftEnviro environmental monitoring software which is a comprehensive solution for visualizing data from mobile monitoring campaigns. It provides a user-friendly interface for collecting, mapping, and analyzing data taken by environmental monitoring technicians while investigating environmental emissions. SyftEnviro provides the tools to identify pollution sources and take immediate action.

This publication reviews VOC pollutant monitoring applications of SIFT-MS in South Korea. SIFT-MS has been applied to emission source characterization, fenceline monitoring, ambient monitoring, pollution mapping, and incident response (including the use of drone-based sampling) for hazardous air pollutants (HAPs), odor nuisance species, and compounds that have high ozone formation potential (OFP) and/or contribute to secondary aerosol (SOA) formation.

HPLC coupled with negative ESI-MS provides a reliable method with unique capabilities for separation and identification of (therapeutic) oligonucleotides.

This application note demonstrates that the Agilent 6546 LC/Q-TOF in Targeted MS/MS acquisition mode is suitable for meeting performance requirements in US EPA Method 533 for the quantitative analysis of PFAS

This application note demonstrates the use of FluoroMatch Suite for the analysis and identification of per- and polyfluorinated substances (PFAS) using an Agilent 1290 Infinity II LC and Agilent 6546 LC/Q-TOF mass spectrometer with iterative exclusion (IE).

This application note describes a rapid analytical method for the detection of 20 perfluoroalkyl substances (PFAS) in human hair within 10 minutes. Solid phase extraction (SPE) using the Agilent Bond Elut ENV cartridge was used to extract the sample