Application Notes: LC
How Method-Development Software Worked for Dow
Dow scientists improved their method-development workflow by upgrading their hardware and using specialized software.
Optimized methods for µPAC™ capLC columns
PharmaFluidics’ μPAC™ capLC is evaluated for different user needs ranging from 12 samples per day in comprehensive mode to 65 samples per day in throughput mode.
High throughput DAR determination of Brentuximab vedotin
YMC’s latest HIC column is used to analyse the DAR of Brentuximab vedotin allowing higher flow rates and shorter run times thanks to the pressure resistant particle.
Analysing Bevacizumab, its aggregates and fragments
The dedicated SEC column for antibodies, YMC-SEC MAB, is used to separate the MAb monomer from its aggregates as well as from degradation products in a single run.
Reverse-phase separation of disulfide modified oligonucleotides
The less hydrophobic wide-pore column YMC-Triart Bio C4 is used for the analysis of modified oligonucleotides due to the hydrophobic character of the disulfide units.
Commercial MAbs by Micro-LC–MS/MS
YMC-Triart Bio C4 is used in YMC capillary column format to analyse and identify intact commercial MAbs by micro-LC- MS/MS at an elevated temperature of 75°C.
Optimisation of phosphorothioate oligonucleotide AEX separation
This application note shows how to achieve narrow peaks and high sensitivity by optimising the AEX separation of phosphorothioate oligonucleotides (PS) in only 5 steps.
Analysis of intact proteins by Micro-LC–MS/MS
In this application note eight intact proteins in the protein mix MSRT2 are analysed by Micro-LC–MS using an YMC-Triart Bio C4 capillary column.
UHPLC analysis of challenging MAbs
This application note using YMC-Triart Bio C4 shows a robust separation at high temperatures of 3 commercially available MAbs: Ofatumumab, Palivizumab, and Panitumumab.
LC–MS compatible ADC analysis by reverse-phase
In this application YMC-Triart Bio C4 was used to analyse a reduced monoclonal antibody and a reduced ADC mimic with formic acid or TFA as additives.
New Gas Chromatography Products for 2019–2020
“GC Connections” presents the column’s annual review of new developments in the field of gas chromatography seen at Pittcon and other venues in the past 12 months.
The Method Development Advantages Offered by Hybrid-Silica Products
Cell Protein Analysis Using Agilent AssayMAP Bravo
An HCP analysis workflow including the Agilent AssayMAP Bravo platform for automated sample preparation, 6545XT AdvanceBio LC/Q‑TOF for LC-MS/MS analysis
ROI and Productivity Increases with Ultivo
This whitepaper demonstrates the features and benefits of owning an Ultivo LC/TQ in terms of actual tangible benefits to your laboratory operations.
Determination of Nitrosamine Impurities Using Ultivo
A method describing the detection of 12 potentially mutagenic nitrosamine impurities found in sartan drugs using the Ultivo LC/TQ
Improve ROI with Integrated LC–MSD Solution
Use interactive calculators included in this whitepaper to determine the incremental economic value of the Agilent 1260 Infinity II Prime LC and InfinityLab LC/MSD iQ.
Ruggedness of YMC Cyano High-Strength Stationary Phase Under Acidic and Basic Extremes
This application note investigates the durability of YMC’s high-strength “HG” Cyano phase under highly acidic and highly basic mobile phase conditions.
Preparative Purification of Cannabidiol from Hemp Oil Extract
This application note details a loading study for purifying cannabidiol (CBD) from a commercially available “high-CBD” hemp oil extract.
Improved USP Chlorhexidine Gluconate Assay on YMC-Triart C18 and YMC Meteoric Core HPLC Packing Materials
This application note details improved HPLC assay methods for chlorhexidine gluconate on hybrid-silica and core-shell C18 stationary phases.
HPLC Separation of Cannabidiol and Melatonin on YMC-Triart C18
This application note details the development of a HPLC method for analyzing CBD and melatonin.
Part 1: Advances in Field-Flow Fractionation: Supporting Development of Novel Nanomedicines
· The benefits of field-flow fractionation · The benefits of coupling light scattering online · Field-flow fractionation’s role in the pharmaceutical industry
Part 2: Advances in Field-Flow Fractionation: Supporting Development of Novel Nanomedicines
Part 3: Advances in Field-Flow Fractionation: Supporting Development of Novel Nanomedicines
An Overview of a Novel Analytical Affinity Chromatography Column
Inert CVD Flow Path Coating for Greater HPLC Sensitivity, Reliability, and Efficiency
Capillary Flow LC–MS Using Micro Pillar Array Columns: Combining Nano Flow Sensitivity with Analytical Flow Robustness and Throughput
-Proteomics based on MS is vital for biopharmaceutical research. -LC–MS instrument sensitivity has led to interest in capillary/microflow solutions. - Microchip-based pillar array chromatography offers enhanced benefits. - Microchip-based pillar array chromatography offers enhanced benefits.
Helpful HPLC Columns You May Not Know About
- Cogent TYPE-C™ HPLC columns offer time and cost benefits to chromatographers. - These columns are suited for polar, non-polar, and water-labile compounds.
LCMS Concentration Studies with RSA Glass
Using RSA™ glass vials virtually eliminates adsorption issue without surface treatments or vial deactivation like silanizing, which can leach into instruments and columns.
UHPLC Analysis of mAbs
This note using YMC-Triart Bio C4 shows a robust separation at high temperatures for three commercially available mAbs: ofatumumab, palivizumab, and panitumumab.
Transfer Method for Impurity Analysis of Chlorhexidine
This application note demonstrates the transfer of analytical HPLC methods from a Waters Alliance HPLC system to the Vanquish Core HPLC system.
