Application Notes: LC
New Gas Chromatography Products for 2019–2020
“GC Connections” presents the column’s annual review of new developments in the field of gas chromatography seen at Pittcon and other venues in the past 12 months.
The Method Development Advantages Offered by Hybrid-Silica Products
Cell Protein Analysis Using Agilent AssayMAP Bravo
An HCP analysis workflow including the Agilent AssayMAP Bravo platform for automated sample preparation, 6545XT AdvanceBio LC/Q‑TOF for LC-MS/MS analysis
ROI and Productivity Increases with Ultivo
This whitepaper demonstrates the features and benefits of owning an Ultivo LC/TQ in terms of actual tangible benefits to your laboratory operations.
Determination of Nitrosamine Impurities Using Ultivo
A method describing the detection of 12 potentially mutagenic nitrosamine impurities found in sartan drugs using the Ultivo LC/TQ
Improve ROI with Integrated LC–MSD Solution
Use interactive calculators included in this whitepaper to determine the incremental economic value of the Agilent 1260 Infinity II Prime LC and InfinityLab LC/MSD iQ.
Ruggedness of YMC Cyano High-Strength Stationary Phase Under Acidic and Basic Extremes
This application note investigates the durability of YMC’s high-strength “HG” Cyano phase under highly acidic and highly basic mobile phase conditions.
Preparative Purification of Cannabidiol from Hemp Oil Extract
This application note details a loading study for purifying cannabidiol (CBD) from a commercially available “high-CBD” hemp oil extract.
Improved USP Chlorhexidine Gluconate Assay on YMC-Triart C18 and YMC Meteoric Core HPLC Packing Materials
This application note details improved HPLC assay methods for chlorhexidine gluconate on hybrid-silica and core-shell C18 stationary phases.
HPLC Separation of Cannabidiol and Melatonin on YMC-Triart C18
This application note details the development of a HPLC method for analyzing CBD and melatonin.
Part 1: Advances in Field-Flow Fractionation: Supporting Development of Novel Nanomedicines
· The benefits of field-flow fractionation · The benefits of coupling light scattering online · Field-flow fractionation’s role in the pharmaceutical industry
Part 2: Advances in Field-Flow Fractionation: Supporting Development of Novel Nanomedicines
Part 3: Advances in Field-Flow Fractionation: Supporting Development of Novel Nanomedicines
An Overview of a Novel Analytical Affinity Chromatography Column
Inert CVD Flow Path Coating for Greater HPLC Sensitivity, Reliability, and Efficiency
Capillary Flow LC–MS Using Micro Pillar Array Columns: Combining Nano Flow Sensitivity with Analytical Flow Robustness and Throughput
-Proteomics based on MS is vital for biopharmaceutical research. -LC–MS instrument sensitivity has led to interest in capillary/microflow solutions. - Microchip-based pillar array chromatography offers enhanced benefits. - Microchip-based pillar array chromatography offers enhanced benefits.
Helpful HPLC Columns You May Not Know About
- Cogent TYPE-C™ HPLC columns offer time and cost benefits to chromatographers. - These columns are suited for polar, non-polar, and water-labile compounds.
LCMS Concentration Studies with RSA Glass
Using RSA™ glass vials virtually eliminates adsorption issue without surface treatments or vial deactivation like silanizing, which can leach into instruments and columns.
UHPLC Analysis of mAbs
This note using YMC-Triart Bio C4 shows a robust separation at high temperatures for three commercially available mAbs: ofatumumab, palivizumab, and panitumumab.
Transfer Method for Impurity Analysis of Chlorhexidine
This application note demonstrates the transfer of analytical HPLC methods from a Waters Alliance HPLC system to the Vanquish Core HPLC system.
U/HPLC Workflow Solution for Biosimilar Characterization
The molecular similarity between Humira® and Yervoy® biosimilars and the corresponding innovator reference products is examined using analytical SEC, RPC and HIC.
Optimize Selectivity Using Fused-Core® Particles
This white paper discusses the advantages of using fast, efficient HALO® Fused-Core® Technology columns and manipulating selectivity to quickly develop methods, for fast high efficiency LC and LCMS separations.
Separating Sugar Isomers by HPLC
Simple sugars ribose and xylose (are easily and quickly separated on a very stable amide column using high acetonitrile concentration in the mobile phase.
Analysis of Mycotoxins in Hemp and Hemp-Containing Edible Products
Assessment of µPAC™ capLC Column Performance
PharmaFluidics introduces a new µPAC™ capLC column that offers unprecedented performance and that given its low back pressure can be used up to flow rates of 15 µL/min.
Simplifying Solid-Phase Extraction for Faster and Cleaner Results
Solid-phase extraction (SPE) is a powerful technique for LC–MS analysis. SPE offers benefits for various solutes from a wide range of samples. Modern SPE systems can remove unwanted compounds and streamline methods.
Separation of Aflatoxins on HxSil C18
Essential oils have been a staple of the food, fragrance, and health markets for many years. Owing to their unique composition, essential oils can be used for a variety of applications.
UHPLC/HPLC Method Development for Pharmaceutical-Related Substance
-How to translate methods, maximize resolution, and comply with USP -Optimizing HPLC/UHPLC method development workflows -Column coupling can provide improved sample detail and high peak capacities
Antibody Analysis with UHPLC-MS
- UHPLC-MS method for antibody analysis - Large-size protein separation at high temp
Editors' Series: Streamlined Characterization of Antibody–Drug Conjugates by Two-Dimensional (2D) and Four-Dimensional (4D) LC-MS
Multidimensional LC/MS is a powerful tool to gain info in the laboratory. Analyzing ADCs with multidimensional LC/MS provides fast data for CQAs.
