To Selectivity and Beyond: Celebrating 18 Years of the Hydrophobic Subtraction Model
In this Thursday morning session, the latest applications of the hydrophobic subtraction model (HSM) will be presented. The session is to be held in Room W181A, and begins at 8:30 am.
In this Thursday morning session, the latest applications of the hydrophobic subtraction model (HSM) will be presented. The session will be held in Room W181A, and begins at 8:30 am.
Dwight Stoll of Gustavus Adolphus College, will kick off the session at 8:30 am with a talk on the use of the HSM to identify columns that are very similar to or different from each other, and the development of a free public database of reversed-phase liquid chromatography (LC) columns.
Next, Richard Henry, an independent consultant, will discuss the use of the HMS to developrugged USP HPLC methods. His talk starts at 9:10 am.
Retention prediction in revesed-phase LC using quantitative structure-retention relationships using the HSM will be presented next, by John Dolan of LC Resources. This talk begins at 9:45 am.
After the recess, Tony Taylor of Crawford Scientific will discuss “Refining the Hydrophobic Subtraction Model using Mathematical Modelling of a Large Data set to Improve LC Column Comparison.” The discussion starts at 10:35 am.
Joe Foley of Drexel University will present the final talk in this session entitled “Are Two Columns Better than One?: Answers from the Hydrophobic Subtraction Model Database and Other Sources.” This talk will be under way at 11:10 am and conclude at 11:45 am.
Hydrophilic Interaction Liquid Chromatography for Oligonucleotide Therapeutics: Method Consideration
March 10th 2025Hydrophilic interaction liquid chromatography (HILIC) has emerged as a promising alternative to traditional ion-pair reversed phase liquid chromatography (IP-RPLC) methods for separating oligonucleotides (ON). This work investigates the application of HILIC to the separation of ON sequence and length variants, duplexes, and single-stranded components.
Matrix Effects on Quantitation in Liquid Chromatography: Sources and Solutions
March 10th 2025The “matrix effect” is one of the sticky details that can make the use of liquid chromatography (LC) for quantitative purposes challenging, even though it is conceptually quite straightforward. In this installment of LC Troubleshooting, Dwight Stoll reviews what is being referred to during talks about “matrix effects”, and discusses some of the potential origins of the phenomenon in practice.
The Next Frontier for Mass Spectrometry: Maximizing Ion Utilization
January 20th 2025In this podcast, Daniel DeBord, CTO of MOBILion Systems, describes a new high resolution mass spectrometry approach that promises to increase speed and sensitivity in omics applications. MOBILion recently introduced the PAMAF mode of operation, which stands for parallel accumulation with mobility aligned fragmentation. It substantially increases the fraction of ions used for mass spectrometry analysis by replacing the functionality of the quadrupole with high resolution ion mobility. Listen to learn more about this exciting new development.
