The Application Notebook
This application note outlines a simple three-step method for the extraction of loperamide and its main metabolite, N-desmethyl loperamide, from blood. UCT’s Clean Screen® XCEL I column provides users with the same level of sample clean-up as traditional SPE while allowing the elimination of timely conditioning and wash steps.
Loperamide is an over-the-counter antidiarrhoeal drug that has been considered to be safe when used as directed. However, as the opioid epidemic continues to ravage our nation there has been an increasing number of reported cases of loperamide overdose. This application note outlines a simple three-step method for the extraction of loperamide and its main metabolite, N-desmethyl loperamide, from blood. UCT’s Clean Screen® XCEL I column provides users with the same level of sample clean-up as traditional SPE while allowing the elimination of timely conditioning and wash steps.
Procedure
Sample Pretreatment
SPE Procedure
Instrumental
LC–MS/MS: Thermo Scientific™ Dionex™ 3000 (LC) TSQ Vantage™ (MS/MS)
Column: UCT Selectra® DA HPLC Column 100 × 2.1 mm, 1.8-μm
Guard Column: UCT Selectra® DA Guard Column 10 × 2.1 mm, 1.8-μm
Injection Volume: 1 μL
Mobile Phase A: D.I. H2O + 0.1% formic acid
Mobile Phase B: Acetonitrile + 0.1% formic acid
Column Flow Rate: 0.30 mL/min
Results
Conclusion
This application note effectively outlines a simple three-step method for the extraction of loperamide and its main metabolite, N-desmethyl loperamide, from blood. UCT’s Clean Screen® XCEL I column provides users with the same level of exceptional sample clean-up as traditional SPE while allowing the elimination of timely conditioning and wash steps in addition to a concentration step. Sample extracts are analyzed via UHPLC–MS/MS utilizing UCT’s Selectra® C18 1.8 µm analytical column.
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