Determination of Aroclor 1248 in Rat Plasma and Liver Matrices: Recovery and Quantification Considerations
The author determines the uncertainty in the quantification of four rat liver samples by applying a statistical evaluation.
Requirements for Validating a Capillary Isoelectric Focusing Method for Impurity Quantitation
The guest authors present a strategy for validating a capillary isoelectric focusing method intended to quantitate impurities in a protein drug substance.
Mobile-Phase Buffers, Part I — The Interpretation of pH in Partially Aqueous Mobile Phases
Guest author G. William Tindall discusses pH measurements in aqueous solutions and the changes that occur when organic solvent is added.
Fused-Silica Capillary — The Story behind the Technology
Guest author Steve Griffin identifies the fundamental problems in capillary manufacturing techniques that cause capillary defects and suggests some corrective actions.
Measuring Column Flow and Velocity
John Hinshaw discusses the measurement of column flow and its interrelationship with carrier-gas velocity.
In Memory: A.J.P. Martin (1910–2002)
Leslie Ettre remembers A.J.P. Martin, inventor of partition, paper, reversed-phase, and gas–liquid partition chromatography.
Practical Considerations of the Influence of Organic Modifiers on the Ionization of Analytes and Buffers in Reversed-Phase LC
This article provides theoretical background, experimental data, and practical considerations about the effects of organic modifiers on analyte ionization in reversed-phase LC.
The Hazards of Adjusting Gradients
John Dolan examines problems associated with adjusting gradients in reversed-phase LC.
To the Editor
Letters about calculating peak asymmetry and gas cylinder safety.
The Retention Behavior of Reversed-Phase HPLC Columns with 100% Aqueous Mobile Phase
The authors studied the retention behavior of reversed-phase HPLC columns under 100% aqueous mobile-phase conditions.
On the Interpretation of GC × GC Data
The authors present their observations from developing data-handling software for GC × GC separations.
Highlights of HPLC 2002
Ron Majors describes developments reported at HPLC 2002.
The Jának-Type Gas Chromatographs of the 1950s
Leslie Ettre describes the development and applications of this long-forgotten unit.
Gas Chromatography at the 25th International Symposium on Capillary Chromatography
John Hinshaw reports about the 25th International Symposium on Capillary Chromatography and Electrophoresis.
Validation of Bioanalytical Capillary Gel Electrophoresis Methods for the Determination of Oligonucleotides
Guest authors describe how this validation is performed routinely in their laboratory.
Performance Qualification of LC Systems
This month's column discusses a performance qualification test designed by PerkinElmer Analytical Instruments (Shelton, Connecticut).
Validation Kits
Product release from NLG Analytical...
Determination of Triglyceride Composition of Vegetable Oils Using High Performance Liquid Chromatography and Evaporative Light Scattering Detection
A fast HPLC method for separating several triglycerides in edible oils with evaporative light scattering detection. (Alltech Associates, Inc.)
Extraction of Low Concentration Levels of 6-MAM from Urine with a Mixed-Mode SPE Sorbent — Strata™ Screen-C
Solid-phase extraction of 6-monoacetylmorphine from urine followed by GC analysis. (Phenomenex Inc.)
Analysis of U.S. EPA Method 8270D — Semivolatile Organic Compounds by GC/MS
Automated SPE in combination with large-volume injection GC–MS analysis of semivolatile compounds. (Horizon Technology, Inc.)
Hemoglobin Characterization
SEC analysis of hemoglobin using multiangle light-scattering detection. (Wyatt Technology Corporation)
"Green" Analysis of Diet Soft Drinks Containing Caffeine and Aspartame
Analysis of several commercial diet soft drinks using HPLC with a 100% water mobile phase. (ZirChrom Separations, Inc.)
Carbamate Pesticide Analysis: Convergence of Micro Postcolumn Derivatization and LC–MS
Pesticide analysis using HPLC with postcolumn derivatization. (Pickering Laboratories, Inc.)
A Reversed-Phase HPLC Column for the Separation and Characterization of Peptides Using LC-UV-MS
Reversed-phase columns retain and separate peptides using MS-friendly mobile-phase additives without compromising peak shape, peak capacity, or resolution. (Waters Corporation)
Mobile Phase Optimization for the Analysis of an Antibody-Based Fusion Protein and Aggregates Using a TSKgel Super SW3000 Size-Exclusion Column
Replacing sodium chloride with sodium perchlorate in the separation of an antibody-based fusion protein provided the robustness of an otherwise problematic separation method. (Tosoh Biosep LLC)
Analysis of triglycerides using HPLC with evaporative light-scattering detection.
Morphine
TLC analysis of morphine. (Analtech, Inc.)
New LC Phase for the Separation of Phospholipids: Pholipidec®
LC analysis of phospholipids. (Advanced Separation Technologies Inc. [astec])
High Throughput Screening — LC/MS with ELS Detection
Analysis of a combinatorial library using evaporative light-scattering detector for concentration detection in an LC–MS system. (Polymer Laboratories, Inc.)
Improved Peak Resolution and Shape for Antidepressants on a New Silica-Based Reversed-Phase Column
Reversed-phase separation of antidepressants on new C18 and C8 silica-based HPLC columns. (Dionex Corporation)
