Embedded-Polar-Group Bonded Phases for High Performance Liquid Chromatography
The authors compare the chromatographic properties of embedded-carbamate-group bonded phases with those of their purely alkyl counterparts.
The Birth of Partition Chromatography
May 1st 2001On the occasion of the 60-year anniversary of the invention of partition chromatography and the 50-year anniversary of the introduction of gas-liquid partition chromatography, this installment of Milestones in Chromatography discusses the circumstances that led to these developments...
Practical Aspects of Mucopolysaccharidoses Screening with Thin-Layer Chromatography
April 1st 2001Scored layers, sequential development, modifications of the development chamber, and a careful choice of standards produce better reliability in thin-layer chromatography analysis of urine for the diagnosis of mucopolysaccharidoses. In equivocal cases, repeating the procedure and varying the solvents will improve results.
New Chromatography Columns and Accessories at the 2001 Pittsburgh Conference, Part I
March 1st 2001The first of a two-part series in which Majors examines the trends in column introductions at Pittcon 2001. He describes HPLC columns and packings for reversed-, normal-, and bonded-phase; ion-exchange; ion; size-exclusion; and large- and preparative-scale chromatography. He also looks at specialty HPLC columns.
Multidimensional Chromatography and the Signature Peptide Approach to Proteomics
This article examines the role that multidimensional chromatography systems will play in high-throughput proteomics.
Analytical Procedures and Method Validation: Highlights of the FDA's Draft Guidance
The FDA recently updated its 1987 guidelines for submitting samples and analytical data for method validation — a document that affects all analytical chemists working in the pharmacrutical industry. This article highlights the agency's recommendations of how to report and validate analytical data in all new drug applications.
New Directions for Concentration Sensitivity Enhancement in CE and Microchip Technology
January 1st 2001Norberto Guzman and Ron Majors look at three areas in which progress has been made in improving concentration limits of detection for CE and CE microchip techniques: preconcentration methods, detector enhancements, and derivatization methods to improve separation selectivity and to enhance detection.