Advances in (U)HPLC

This article compares the performance of wide-pore silica monolithic, sub-2µm FPP, and SPP columns, addresses the question of whether 1000 Å or 400 Å SPP columns are more suitable for reversed-phase LC-type protein separations, and presents a kinetic performance comparison of different columns.

A micro-pillar array format for mapping the proteome of human stem cell-derived liver organoids using timsTOF–MS is presented.

This article highlights an advantage of porous graphitic carbon columns, that is their resistance at high temperatures, for the analysis of parabens in foodstuffs.

An introduction from guest editor, Gert Desmet from Vrije Universiteit Brussel, Belgium

The occurrence of convex-upward van Deemter curves is rare but not unusual in chiral liquid chromatography. In this work, this behaviour, experimentally observed for the more retained enantiomer of a chiral sulfoxide on a polysaccharide-based CSP, is explained.

A recent trend in the design of LC instrumentation is the move towards miniaturized and portable systems.