July / August 2023

Trypsin is one of the most commonly used proteases in peptide mapping protocols because of its high level of specificity. However, trypsin alone is not always sufficient for full sequence coverage. In this article, the authors detail how trypsin was combined with chymotrypsin to overcome this, and the benefits of an automated platform.

How to design scouting gradients, how to use the resulting data to make decisions about next steps, and how to improve the separation once an elution mode has been chosen.

This instalment explores the challenges involved in detecting the effluent from a capillary gas chromatographic column and summarizes the many detector options.

An overview of different approaches for the qualitative and quantitative analysis of polysorbates.

On a fundamental level, equilibrium and diffusion define chemical separations.

ISSS 2023 and other important events for chromatographers.