March 2025

In this first roundtable discussion focused on Women in Chromatography, LCGC International spoke with Diane Turner, Jacqueline Hamilton, and Caroline Gauchotte-Lindsay, who discussed emerging trends in chromatography and useful resources for women in chromatography.

In this first roundtable discussion focused on Women in Chromatography, LCGC International spoke with Diane Turner, Jacqueline Hamilton, and Caroline Gauchotte-Lindsay, who offered their advice to young women looking to enter the chromatography field.

In this first roundtable discussion focused on Women in Chromatography, LCGC International spoke with Diane Turner, Jacqueline Hamilton, and Caroline Gauchotte-Lindsay about how the representation of women in chromatography has evolved and what changes still need to be made.

In this first roundtable discussion focused on Women in Chromatography, LCGC International spoke with Diane Turner, Jacqueline Hamilton, and Caroline Gauchotte-Lindsay about what inspired them to pursue a career in chromatography and what challenges they faced along the way.

In this first roundtable discussion focused on Women in Chromatography, LCGC International spoke with Diane Turner, Jacqueline Hamilton, and Caroline Gauchotte-Lindsay, who are all leading voices in the LinkedIn International Women in Chromatography group.

From June 12–13, 2025, the 3rd Advances in Separation Science Workshop will take take place in Gembloux, Belgium.

Here's a taster of what to look forward to at the 54th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2025), which will be held from Sunday through Thursday, 15–19 June, 2025, in Bruges, Belgium.

LCGC International spoke with Daniel Meston and Dwight Stoll from Gustavus Adolphus College in St. Peter, Minnesota, USA, about a project they worked on with Todd Maloney from Eli Lilly in Indianapolis, Indiana, USA, to investigate the optimal performance conditions of antisense oligonucleotides (ASOs) when using hydrophilic interaction liquid chromatography (HILIC).

Sample concentration is a parameter that can influence the quality of gel permeation chromatography/size-exclusion chromatography (GPC/SEC) separations and the obtained results. Understanding this influence can help to support the development of reliable GPC/SEC methods.

This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.

This article discusses several practical examples using bioinert columns, demonstrating their ability to deliver reliable, high-resolution results with precision and reproducibility.

How is the indoor air affecting your health?

The authors present their motivation for establishing the Young Scientists of FFF (YSFFF) initiative within the FFF community.

What’s the next step in your career?

A pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) methodology has been specifically developed for the identification and quantification of seven polymers commonly found in tap water. The researchers responsible for the approach state that it prioritizes both time and cost efficiency without compromising the thoroughness of marker spectrum detection and confirmation.

A two-step workflow to extract pure mass spectra from comprehensive two-dimensional liquid chromatography high-resolution mass spectrometry (LC×LC–HRMS) data has been created and tested.

A new method has been created for determining N-nitrosamines (NAs) in pharmaceutical preparations using liquid chromatography with tandem mass spectrometry.

Click here to view the Europe interactive link of The Column March 2025 issue.

Click here to view the North America interactive link of The Column March 2025 issue.

Chromatography companies recognize significant opportunities in the biotech and pharma sectors, yet face uncertainty driven by rising tariffs and the growing integration of AI tools.