Application Notes

When scaling an established analytical method across columns packed with different particle sizes and different column configurations (internal diameters and lengths), the amount of time that is required outside of the lab to produce the equivalent method conditions is considerable. When dealing with a gradient method, the calculations required include determining the new flow rate, gradient times, and injection volume. An analyst can perform these calculations manually using the appropriate equations or tools like the Waters Columns Calculator. This app note examines two scaling workflows by first performing a theoretical scale-down experiment manually using the appropriate method scaling equations, and then repeating the experiment using the scaled-down conditions generated by the Waters Columns Calculator. Strong agreement between manual calculations and the results using the Waters Columns Calculator validate the Waters Column Calculator for its use in scaling methods effectively, with a significant improvement in time savings and a reduction in potential calculation errors and/or uncertainties.

Pro EZLC online tools make it easy to develop and optimize new LC methods or translate existing ones quickly and accurately. See the effects of parameter changes instantly at your desk without spending time in the lab or tying up an instrument. Take advantage of Restek’s years of chromatographic expertise at any time, from anywhere, with simple-to-use yet incredibly powerful EZLC method development tools.

To address the Helium shortage GC laboratories have begun looking at possible replacement gases and new approaches to help obtain the carrier gases needed to continue their work. One of the most promising options is using the use of gas generators to provide a steady supply of hydrogen gas as a replacement for helium. Although some concerns—and some myths—persist about using hydrogen, it offers multiple benefits and advantages over helium, notably particularly when a gas generator replaces high-pressure gas cylinders.

A Methods-Based Reference to Lab Supplies for PFAS Testing

Hydrogen is the perfect molecule for gas chromatography, especially for the replacement of the expensive Helium.

Discover the GC 2400™ Platform for analytical precision and robustness for fuel alcohol analysis over a wide range of sample concentrations.

Master Gas Chromatography Troubleshooting - Streamline Your Analysis with Expert Guidance.

Discover how the PerkinElmer GC 2400™ Platform provides fast analysis and high reproducibility.

Discover how the PerkinElmer GC 2400™ Platform helps with the residual solvent in plastic with accuracy and sensitivity, providing reliable and reproducible runs for high throughput laboratories.

Discover how the PerkinElmer GC 2400™ Platform helps improve resolution requirements.

Discover an optimized GC/MS approach for SVOC in complex matrices.

Read how the integrated HS-GC workflow allows a continuous optimization of the pneumatic values for ultimate precision in GC retention time.

Inside the Laboratory is a joint series with LCGC International and Spectroscopy, profiling analytical scientists and their research groups at universities all over the world. This series spotlights the current chromatographic and spectroscopic research their groups are conducting, and the importance of their research in analytical chemistry and specific industries. In this “Inside the Laboratory –– A Look at Environmental Analysis, PFAS, and Contaminants of Emerging Concern” special edition PDF, we profile Dr. Lee Blaney and his laboratory at the University of Maryland Baltimore County (UMBC), in Baltimore, Maryland. This special edition PDF highlights the research Dr. Blaney is spearheading in his laboratory, which include studying contaminants of emerging concern and developing advanced analytical methods that can better improve detection and quantification of CECs in the environment, as well as producing new treatment technologies for diverse contaminants in water and wastewater.

This note summarizes the optimal methods for the HPLC separation of semaglutide and liraglutide under acidic and alkaline conditions.

This application note discusses how to streamline method transfer outcomes using ACD/Labs' method development software, Method Selection Suite.

Discover the supremacy of the bioinert coated hardware of YMC Accura BioPro IEX QF columns for reproducible analysis of oligonucleotides in ion-exchange chromatography.

This application note demonstrates the use of SCION's 8500GC system for the analysis of key greenhouse gases—carbon dioxide, methane, and nitrous oxide—in a single atmospheric air matrix. Highlighting the system's excellent sensitivity and repeatability, this method is crucial for understanding emission sources and combating climate change.

This application note outlines a method for detecting low levels of 1,4-Dioxane, a likely carcinogenic compound, in drinking water using SCION GC-MS technology. It details the procedure for analyzing water samples spiked with 1,4-Dioxane, achieving excellent sensitivity and low detection limits, demonstrating the method's effectiveness for environmental monitoring.

This application note from SCION Instruments delves into the meticulous screening of impurities in plant protection products using GC-FID, with GC-MS for confirmation. It emphasizes the necessity of identifying and quantifying impurities to comply with regulatory standards in industries such as pharmaceuticals, food, and agriculture. The study specifically examines eugenol, showcasing how to determine significant impurities for regulatory submission. The approach integrates GC-MS and analytical standards, ensuring precise impurity identification and quantification critical for product approval.

We show the development of HPLC-ELSD methods for high-throughput and sensitive analysis of the lipids in lipid nanoparticles (LNPs), used to administer mRNA vaccines.

Recycling chromatography is a powerful technique that allows the separation of two closely eluting peaks by increasing the column bed length.

KNAUER AZURA UHPLC system, thanks to the low dispersion and operating pressure up to 1240 bar, allows to speed up your method and increase throughput preserving resolution

This application note showcases the SCION 8300 GC with SQ MS's capability to detect limonene in recycled HDPE pellets, emphasizing its high sensitivity and robustness. The method ensures that the recycled pellets, crucial for manufacturing deodorized food packaging, contain limonene concentrations well below the 20ppm threshold, demonstrating the efficacy of the recycling process.

Trifluoroacetic acid (TFA) as an eluent modifier causes baseline ripples, with negative effect on LOD/LOQ. This can be mitigated by adjusting the pump mixer volume

The KNAUER IJM NanoScaler is used to precipitate protein metal carbonate hybrid particles by mixing two inorganic salt solutions in which the protein is dissolved

The KNAUER HPLC Method Converter assists you to modernize the analytical method according to the allowed variations last US and EU Pharmacopeia (USP) chapter 621

Explore SCION Instruments' method for simultaneously analyzing two triazole fungicides, Paclobutrazol and Uniconazole, using HPLC-DAD. This Application Note provides insights into the procedure's effectiveness, highlighting its importance for environmental labs due to health and regulatory reasons.

A preparative system setup is presented for the purification and fractionation of target substances at flow rates up to 800 ml/min with a max. back pressure of up to 100 bar.

Clinical trials can be helpful indicators of where the life sciences industry is headed. Dean Erhardt, BBA, MBA, President & CEO of D2 Solutions, offers his insights regarding the direction of the market and potential growth areas.