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LC–MS Analysis of Oligonucleotides v2
Oligonucleotides were then analyzed using liquid chromatography with ultraviolet and mass spectrometry with a polymer-based diol column.
Collaborative Validation of a New Method
SGS AXYS and Waters validated GC–MS/MS as an alternative procedure to HRMS, and adapted EPA Method 1613B criteria, proving equivalency in results and performance metrics.
Determination of PFAS in drinking water
This application note demonstrates a reliable SPE method with an automated SPE system for the determination of PFAS in drinking water per U.S. EPA Method 533.
PFAS in drinking water using SPE
Reliable SPE for PFAS in drinking water per U.S. EPA Method 537.1
Analysis of Sugars in Syrup by HPLC
This application note presents a simple HILIC-RI method for the analysis of four common sugars found in maple syrup, utilizing an LC 300 HPLC with Refractive Index detector.
Analysis of Preservatives in Cosmetics by HPLC
This application demonstrates a fast, robust, and sensitive HPLC method for the analysis of eight parabens in commercially available cosmetic and personal care products.
Analysis of Isoflavones in Supplements by HPLC/UV
Efficient and reliable UHPLC method for the analysis of 12 isoflavones, prepared in accordance with the USP monograph for soy, using an LC 300 with PDA detector.
Trans-galactooligosaccharides in foods using HPAE-PAD
AOAC Method 2001.02 for TGOS analysis executed with a Thermo Scientific Dionex CarboPac PA1-1mm column using HPAE-PAD in Dual EGC Mode.
AOF by combustion IC - PFAS
AOF by combustion IC – non-targeted complemental determination of PFAS in aqueous samples
Extraction and analysis of PFAS from soil
This application note demonstrates how accelerated solvent extraction (ASE) can extract a variety of PFAS from soil.
HPAE-PAD of Galactosyl-Oligosaccharide-Containing Samples
This application note demonstrates dual eluent generation cartridge performance for profiling galactosyl-oligosaccharides using HPAE-PAD.
Sorbitol determination in food
Thermo Scientific Dionex CarboPac PA300-4µm column allows simplified, high-resolution sorbitol separation and quantification in complex samples.
Analysis of 16 Cannabinoids by HPLC/PDA
This work describes a simple and fast method for the analysis of sixteen commonly analyzed cannabinoids utilizing a PerkinElmer LC 300 HPLC with PDA detector.
Agave syrup carbohydrate analysis using HPAE-PAD
This application note demonstrates how Norma Oficial Mexicana for agave syrup carbohydrate analysis is executed with a Thermo Scientific Dionex CarboPac PA1-1 mm column using HPAE-PAD in EGC mode.
Avantor® Chromatography Solutions for Nitrosamine Analysis
Nitrosamines are potentially dangerous genotoxic compounds that have been recently found within some pharmaceutical products. We demonstrate highly sensitive methods to accurately detect and quantify these compounds.
Pharmaceutical Quality Control: The Reference Standards Labyrinth (NA)
Guide to LC Troubleshooting 2021
Analysis of Pesticides in Spinach
The efficacy of the JMS-TQ4000GC triple-quadrupole GC–MS/MS for measuring pesticides in spinach purchased from local grocers is described in this application note.
Analysis of Pesticides in Honey
The efficacy of the JMS-TQ4000GC triple-quadrupole GC-MS/MS for measuring pesticides in honey purchased from local apiaries is described in this application note.
Analysis of Pesticides in Kale
The efficacy of the JMS-TQ4000GC triple-quadrupole GC-MS/MS for measuring pesticides in kale purchased from local grocers is described in this application note.
Molecular weight profiling of oligonucleotides
Combining high-resolution size exclusion chromatography columns with highly sensitive MALS detection characterizes oligonucleotides and structurally-related impurities.
Avoiding Depurination During Trityl-on Purification
This technical note explores a new trityl-on procedure that minimizes the risk of depurination.
Confident Drug Metabolite Identification
This application note presents a case study for metabolite profiling of model pharmaceutical compounds using HRAM MS and small molecule ID software for data processing.
Clarity OTX Application Guide
Extraction of oligo therapeutics from biological samples for LC–MS bioanalysis
Extraction of 2’-MOE Phosphorothioate from Plasma Using Clarity OTX
Using the Clarity OTX extraction protocol vendor recommendations, we have demonstrated its use to develop a method for the extraction of therapeutic oligos from plasma.
App Note: Integrating the Analysis of Ultrashort-Chain PFAS
Method development for simultaneous analysis of ultrashort-chain, alternative, and legacy per- and polyfluoroalkyl substances (PFAS).
The Most Cost-Effective Makeup Gas for GC
Historically, helium has been used as a makeup or carrier gas. Learn about the disadvantages of helium and why so many laboratories are switching to nitrogen gas for GC.
RNA Sample Preparation and High Throughput Purification for TBDMS and TOM Chemistries
This technical note discusses 2' cleavage and RNA sample preparation prior to trityl-on Clarity QSP cartridge or high-throughput purification.
Automated Method Development of Oligonucleotide in Tissues Using Clarity OTX 96-Well Plate and High-Resolution Mass Spectrometry
As synthetic oligonucleotide therapeutics traverse the drug-development path, there is an increasing need for TK and PK/PD studies in drug discovery and development.
FAQs for the Isolation and Purification of Synthetic Oligonucleotides
Common frequently asked question for oligonucleotides. This includes questions about characterization to purification and from sample preparation to LC separation.