Application Notes

HPLC chromatographic methods can be greatly improved and modernized by simply reducing the column’s length and particle size. This not only saves time, but increases consumption and waste generation. By using modern HALO®Fused-Core® column technology, methods can be shortened thereby increasing laboratory efficiency while keeping back pressures under instrument pressure limitations.

HPLC and LC-MS separation materials and methodologies have evolved over the years to meet the challenges of the growing complexity of the separations themselves. In this Q&A, Stephanie Rosenberg, Director of Sales and Marketing discusses how utilizing the new HALO® positively charged surface columns impact the perforance of LC and LCMS separations for basic compounds.

This guide will discuss improved reversed phase biomolecule separation solutions for monoclonal antibodies, as well as chromatography columns used to develop an understanding of the protein backbone via peptide mapping. Glycosylation analysis using HILIC will also be discussed. Examples will highlight areas in the workflow where HALO® Fused-Core® columns with appropriate pore sizes and phases offer unique advantages.

A method is presented for the analysis of the chain-length distribution of inulin-type fructans with degree of polymerization up to 65 using SweetSep™ AEX200 column.

Separation and quantification of the 15 most commonly occurring carbohydrates in honey within 25 min is presented using Antec Scientific ALEXYS™ Carbohydrate Analyzer.

This application note outlines a QuEChERS method for the simultaneous analysis of cannabis for 67 pesticide and 5 mycotoxin residues in cannabis flower.

Antec Scientific developed a new column for the superior separation of all classes of carbohydrates using HPAEC with PAD or MS detection.

Antec Scientific ALEXYS Carbohydrate Analyzer is the most dedicated HPAEC-PAD instrument for the analysis of all classes of carbohydrates.

This application note explores the benefits of using LNI lab benches to enhance the performance of LC–MS systems.

Fast and sensitive analysis of lactose and isomers in ‘lactose-free’ labelled products using Antec Scientific SweetSep™ AEX200 HPAEC column.

The sensitive analysis of common mono– and disaccharides added to meat and fish products is demonstrated using the ALEXYS™ Carbohydrate Analyzer and SweetSep™ column.

This application note demonstrates the performance of SCION's GC combined with FID, showcasing its higher sample throughput.

Automated high-capacity sorptive extraction with GC–MS for screening wine, at ppb levels, for two yeast-derived spoilage compounds in wine.

Today’s SFC systems are widely accepted for analytical and preparative separation of chiral and achiral compounds related to pharmaceutical development. Shimadzu enhances the user experience by delivering a highly effective integration of analytical and prep SFC supported by a unified software platform. Learn how these solutions can be used for rapid screening of multiple analytical conditions, simplified method development, and purification at laboratory scale.

Whether you're an experienced user of SFC technology or just getting into it, this FAQ guide offers a wealth of knowledge to enhance your efficiency, troubleshoot common issues, and make the most of this dynamic tool. Learn about a wide range of topics, including hardware insights, advantages of SFC, applications, and column screening and matrix effects.

SFC can provide solutions for challenging separations beyond traditional normal- and reverse-phase HPLC. But to get the best results, you need to select the right columns. This eBook examines column options for accurately and efficiently separating a variety of pharmaceutical compounds using SFC. Topics include SFC column fundamentals and considerations, selecting SFC columns, and columns for SFC chiral and achiral analysis.

In gas chromatography (GC) analysis, the use of proper high-purity gases is critical to the speed and accuracy of analysis. Laboratories worldwide are taking control of their gas supply with onsite gas generation as a safe and economical way to stabilize gas costs while improving the speed of analysis. LCGC recently sat down with Rod Wilson, Product Manager of Parker Hannifin’s Analytical Gas Business, to discuss the advantages of Parker gas generators.

The Nexera UC UHPLC/SFC switching system combines both UHPLC and SFC analysis modes in a single system, allowing you to easily switch between the two techniques in as few as ten minutes without reconnecting flow lines. So, you get the added resolution of UHPLC and the added selectivity of SFC to quickly develop separations without compromising chromatographic accuracy. Learn about five key benefits.

Accurate purity assessment of oligonucleotide mixtures can be challenging due to their complexity. Daicel offers several solutions that provide unique selectivity.

This application note emphasizes the assessment criteria and key factors to consider when evaluating and choosing chromatographic methods.

The measurement of adenosine nucleotides represents important diagnostic measures concerning metabolic standing for the determination of various pathological states.

The coagulation benefits of vitamin K1 are well known, however, the other congeners of the vitamin series are not as common (K2, K3, and K4), but equally contribute to a healthy disposition.

Plasticizers added to water bottles to add stability to the bottles can be unknowingly extracted in drinks leading to possible endocrine interactions.

In this application note, the charge variant characterisation of an IgG4-based mAb was performed using a BioPro IEX QF anion exchange column coupled to an MS detector.