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Raman Spectroscopy for Streamlining Testing and Reducing Costs in Pharma QC and Formulation Development
• Accelerated compliance for less expense. • Raman spec streamlines non-destructive processes for production/release
EPA 525.2 by Empore™ SPE Disks
This application note demonstrates the performance of 3M™ Empore™ (now CDS Empore™) C18 SPE disks in the monitoring of drinking water samples by EPA Method 525.2.
mAb Method Using Isopropanol in HIC
This application note shows how to achieve higher recoveries for commercially available MAbs using 15 % isopropanol as modifier in HIC mode.
SEC Analysis of mAb Fragments
The analysis of intact MAb and their fragments using the UHPLC compatible YMC-Pack Diol-200 column and specifically designed YMC-SEC MAB column is described.
Reaction Monitoring of ADCs
The effective separation of intact antibodies from ADCs with their single DARs using hydrophilic interaction chromatography linked to UV/Vis is shown.
Generic Analyses of mAbs with Reversed-Phase (U)HPLC
This application note demonstrates how to achieve robust chromatographic results for commercially available MAbs with higher sensitivity and sharper peaks at 80°C.
AEX Separation of Oligonucleotides
In addition to existing RP based methods, a method is presented for the determination of oligonucleotides using YMC’s strong, non-porous anion exchanger BioPro IEX QF.
High Temperature Reversed-Phase Analyses of mAbs
This application note shows how to achieve robust chromatographic results for two commercially available MAbs using a modern temperature-stable widepore RP-phase.
Sensitive LC–MS/MS Analysis of Sphingophospholipids
The application shows that by implementing a metal-free column hardware any tailing caused by phosphate groups is avoided.
Challenging Analysis of Amyloid β (Aβ) Proteins
The method development for the simultaneous determination of highly hydrophobic Amyloid β peptides is described.
Polymer branching analysis by SEC-MALS-IV
Branching impacts polymer properties such as rheology and stiffness. This note explains the analysis of branching ratio vs. molar mass, accomplished by SEC-MALS( -IV).
SEC-MALS for absolute biophysical characterization
SEC-MALS analyzes proteins, peptides, nucleic acids, polysaccharides and bionanoparticles to determine molar mass, size, conjugation, aggregation and interactions.
Applying High-Resolution and High-Efficiency Size Exclusion Chromatography Technology to Biotherapeutic Characterization
• Challenges and solutions for getting the most accurate data from SEC • Combining SEC with UV or MS detection to quickly analyze samples
Analysis of Formoterol, Budesonide, and Related Compounds by UPLC–MS
Explore the analytical potential of the AQbD approach for achieving high-performance separations of formoterol, budesonide, and its related compounds.
Increasing Recovery of Trace-Level Fatty Acid Analytes
The benefits of using QuanRecovery Vials for trace analysis of fatty acids in RPLC-MS based workflows.
Automated Analytical Cation-Exchange Chromatography
In this application note, infliximab and trastuzumab were separated on a BioResolve SCX mAb Column, and their charge variant fractions were collected using a Waters Fraction Manager-Analytical (WFM-A).
A Method for the Quantitative Determination of Pesticide Residues
The development and validation of a robust, quantitative method for the routine determination of a wide range of LC-amenable pesticides following QuEChERS (DisQuE dispersive sample preparation) procedure and LC-MS/MS
HRMS System Check for Intact Protein Quantification
This document is designed to help the user performing intact and/or subunit protein analysis evaluate the performance or suitability of the instrument by undertaking a simple system check (SC) and running of QC standards.
Vivaspin®: Concentration to a Defined Volume
Describes a method for reproducible defined final volumes using several Vivaspin® centrifugal concentrators: Turbo 15, Turbo 4, and 500.
Desalting and Buffer Exchange with Vivaspin® Centrifugal Concentrators
Outlines desalting or buffer exchange processes for Vivaspin concentrators to allow a significant reduction in salt concentration without diluting the protein sample.
Vivaspin® Concentrators for Low-Concentrated Protein Samples
Outlines a pre-treatment process for Vivaspin concentrators to improve recovery of very dilute protein samples in the nano- to microgram concentration range.
A Revolutionary Approach to Light Scattering Technology
• Enhanced sensitivity and range are needed to obtain molecular weight and size. • New light scattering detector addresses flaws of traditional approaches.
