Application Notes: LC

This application is an alternative HPLC method for the analysis of 13 carbonyls without the use of any derivatization agent. The method uses the combined polar and non-polar selectivity of a Kinetex Biphenyl stationary phase to achieve improved chromatographic results.

For this analysis, we developed a fast, robust, and efficient separation on a Kinetex 2.6µm Polar C18 phase. This is a highly robust, core-shell stationary phase capable of providing increased efficiency over fully porous products. The Kinetex Polar C18 functionality provides unique polar selectivity that was critical in effectively retaining and separating these phenolic antioxidants.

This application is an alternative HPLC method for the analysis of 13 carbonyls without the use of any derivatization agent. The method uses the combined polar and non-polar selectivity of a Kinetex Biphenyl stationary phase to achieve improved chromatographic results.

This method presents the reversed-phase retention and detection of 7 polar Neonicotinoids and 4 deuterated-internal standards by LC-MS/MS and with a Kinetex 2.6 Biphenyl HPLC column. The Kinetex Biphenyl phase demonstrated significant polar compound retention and selectivity.

For Developed is a rapid method analyzing 16 major cannabinoids with great resolution and low backpressures.

A HPLC/UHPLC poster that ranks different stationary phases by 5 primary mechanism of reversed-phase selectivity.

Described in this application note are the details for an investigation of basic compound peak shape across four different alkyl C18 stationary phases that are all based upon the same core-shell HPLC/UHPLC particle morphology.

In this application, is the investigation into the relative selectivity of four different C18 stationary phases when applied to the reversed phase retention of six extremely polar artificial sweeteners.

Analysis of paralytic shellfish toxins using a HPLC single step-gradient method with products of post-column derivatization detected using a fluorescence detector

The advantage of UV spectroscopy as an analytical method to detect protein aggregation is that it is non-destructive and uses low sample volumes, minimal sample preparation requirement and easy sample analysis. A quick QC method to indicate the presence of aggregation uses an Agilent Cary 60 UV-Vis spectrophotometer to identify aggregation of mAb solutions resulting from different stress conditions.

Decrease analysis time for fluorochemicals with no sacrifice in chromatographic performance. Rapid perfluorinated alkyl analysis by LC-MS/MS increases sample throughput.

Decrease analysis time for fluorochemicals with no sacrifice in chromatographic performance. Rapid perfluorinated alkyl analysis by LC-MS/MS increases sample throughput.

The Agilent 1290 Infinity II UHPLC, an Agilent 6545 Q-TOF-MS, and Agilent MassHunter BioConfirm B.08.00 software was used for accurate disulphide bond mapping.

An isocratic HPLC method showing the separation of an artificial sweetener in a cola beverage. The chromatogram shows the advantage of using 230nm instead of 254nm for improved detection but minimizing the baseline interferences.

With the increase of research with Eudragit, consistent molecular weight measurements have become more prevalent. Shodex has shown a repeatable SEC method using the GF-510 HQ column.

TSKgel® UP-SW3000 columns are 2 µm SEC columns designed for the analysis of monoclonal antibodies and other biopharma products and can be used on both HPLC and UHPLC systems. A TSKgel UP-SW3000 column yielded very low percent relative standard deviation (%RSD) for peak parameters including retention times, peak asymmetry, and efficiency, demonstrating the exceptional reproducibility of this column versus a competitor UHPLC column.

The characterization of monoclonal antibodies is a major challenge in process monitoring and quality control. TSKgel® G3000SWXL columns have been the industry standard for quality control of mAbs by SEC for decades. With the introduction of TSKgel UP-SW3000, 2 µm silica-based UHPLC–HPLC columns, increased speed and higher resolution can be achieved for the separation of antibody fragments, monomers, and dimers.

This application note discusses the use of a 3 μm particle size, 30 nm pore size, size exclusion chromatography (SEC) column for monitoring folded (native) and unfolded (denatured) states of a monoclonal antibody using fluorescence detection (FLD).

Successful separations of the enantiomers of 3,3’-substituted BINOLs and their corresponding protected derivatives were obtained on the RegisPack® chiral stationary phase (CSP) from Regis Technologies, Inc.

This Application Note proves equivalency of the Agilent 1200 Series LC and the Agilent 1260 Infinity II LC for the analysis of tricyclic antidepressant drugs.

This Application Note proves equivalency of the Agilent 1200 Series LC and the Agilent 1260 Infinity II LC for the analysis of tricyclic antidepressant drugs.

Analysis of arsenic species is made challenging due to diverse sample matrices. To circumvent these problems, an HPLC ICP-MS method has been developed. Resolution of arsenic compounds is achieved by ion exchange chromatography on a Hamilton PRP-X100 column, a 55% cross-linked polystyrene-divinylbenzene copolymer functionalized with quaternary ammonium anion-exchanger group.

Pickering Laboratories, Inc. offers a complete solution for Amino Acids Analysis according to European Pharmacopoeia 8.0. This includes the Pinnacle PCX post-column derivatization instru¬ment, analytical columns and GARDs, buffers and Trione Ninhydrin reagent. The methods presented in this application note were optimized to comply with system suitability requirements of Pharmacopoeia 8.0 methods.

Pickering Laboratories, Inc. offers a complete solution for Amino Acids Analysis according to European Pharmacopoeia 8.0. This includes the Pinnacle PCX post-column derivatization instru¬ment, analytical columns and GARDs, buffers and Trione Ninhydrin reagent. The methods presented in this application note were optimized to comply with system suitability requirements of Pharmacopoeia 8.0 methods.

Monitoring of Glyphosate in crops and water is mandated in many countries. Pickering offers a sensitive and robust HPLC method for analysis of Glyphosate in soy beans, corn and sunflower seeds. This method utilizes a simplified sample preparation procedure that has proven to be effective even for challenging matrices.

Monitoring of Glyphosate in crops and water is mandated in many countries. Pickering offers a sensitive and robust HPLC method for analysis of Glyphosate in soy beans, corn and sunflower seeds. This method utilizes a simplified sample preparation procedure that has proven to be effective even for challenging matrices.

A CHIRALPAK IG column (immobilized meta selector) was used to develop the enantioselective separation of methylclothiazide. Meta-substituted immobilized chiral selectors have been shown to have remarkable affinity for resolution of chiral compounds from different types of molecules. Available in 3 and 5 micron.

A CHIRALPAK IG column (immobilized meta selector) was used to develop the enantioselective separation of methylclothiazide. Meta-substituted immobilized chiral selectors have been shown to have remarkable affinity for resolution of chiral compounds from different types of molecules. Available in 3 and 5 micron.

The quick capture and accurate titer analysis over a wide concentration range of monoclonal antibody is demonstrated using a TSKgel® Protein A-5PW, 20 μm analytical column.

The Agilent 1290 Infinity II LC was used to determine the bitter compounds iso-alpha-acids and reduced iso-alpha-acids, and the clove-like phenolic flavor 4-vinylguaiacol in bottled beer.