Application Notes: LC
>Application Notes: LC
Separation of Vitamin E Congeners by LC–UV Using a Kinetex 2.6 µm F5 Core-Shell LC Column
August 22nd 2019A straightforward separation of 10 closely related forms of vitamin E with a Kinetex 2.6 µm F5 HPLC column and under reversed-phase conditions. The 10 closely related structures were chromatographically separated by the multiple selectivity interaction mechanisms of the Kinetex F5 phase and Water/Methanol mobile phase.
Rapid Analysis of Neonicotinoids by LC–MS/MS Using a Kinetex 2.6 µm Biphenyl Core-Shell LC
August 22nd 2019This method presents the reversed-phase retention and detection of 7 polar Neonicotinoids and 4 deuterated-internal standards by LC-MS/MS and with a Kinetex 2.6 Biphenyl HPLC column. The Kinetex Biphenyl phase demonstrated significant polar compound retention and selectivity.
Effectiveness of Polar Stationary Phase Modification on Peak Shape for Basic Compounds
August 22nd 2019Described in this application note are the details for an investigation of basic compound peak shape across four different alkyl C18 stationary phases that are all based upon the same core-shell HPLC/UHPLC particle morphology.
Comparison of the Reversed-Phase Retention of Six Extremely Polar Artificial Sweeteners
August 22nd 2019In this application, is the investigation into the relative selectivity of four different C18 stationary phases when applied to the reversed phase retention of six extremely polar artificial sweeteners.
Extraction and Separation of Phenolic Antioxidants from Edible Oils
August 22nd 2019For this analysis, we developed a fast, robust, and efficient separation on a Kinetex 2.6µm Polar C18 phase. This is a highly robust, core-shell stationary phase capable of providing increased efficiency over fully porous products. The Kinetex Polar C18 functionality provides unique polar selectivity that was critical in effectively retaining and separating these phenolic antioxidants.
Separation of Vitamin E Congeners by LC–UV Using a Kinetex 2.6 µm F5 Core-Shell LC Column
August 22nd 2019A straightforward separation of 10 closely related forms of vitamin E with a Kinetex 2.6 µm F5 HPLC column and under reversed-phase conditions. The 10 closely related structures were chromatographically separated by the multiple selectivity interaction mechanisms of the Kinetex F5 phase and Water/Methanol mobile phase.
The Analysis of the Polar Base Berberine
August 22nd 2019For this application study, the alkaloid compound berberine was selected to help demonstrate the Kinetex PS C18 HPLC/UHPLC column’s unique multi-modal selectivity and improved chromatographic performance when applied to the analysis of polar basic compounds.
The Analysis of the Polar Base Berberine
August 22nd 2019For this application study, the alkaloid compound berberine was selected to help demonstrate the Kinetex PS C18 HPLC/UHPLC column’s unique multi-modal selectivity and improved chromatographic performance when applied to the analysis of polar basic compounds.
Underivatized Reversed Phase Retention and Separation of 13 Carbonyl Compounds
August 22nd 2019This application is an alternative HPLC method for the analysis of 13 carbonyls without the use of any derivatization agent. The method uses the combined polar and non-polar selectivity of a Kinetex Biphenyl stationary phase to achieve improved chromatographic results.
Extraction and Separation of Phenolic Antioxidants from Edible Oils
August 22nd 2019For this analysis, we developed a fast, robust, and efficient separation on a Kinetex 2.6µm Polar C18 phase. This is a highly robust, core-shell stationary phase capable of providing increased efficiency over fully porous products. The Kinetex Polar C18 functionality provides unique polar selectivity that was critical in effectively retaining and separating these phenolic antioxidants.
Underivatized Reversed Phase Retention and Separation of 13 Carbonyl Compounds
August 22nd 2019This application is an alternative HPLC method for the analysis of 13 carbonyls without the use of any derivatization agent. The method uses the combined polar and non-polar selectivity of a Kinetex Biphenyl stationary phase to achieve improved chromatographic results.
Rapid Analysis of Neonicotinoids by LC–MS/MS Using a Kinetex 2.6 µm Biphenyl Core-Shell LC
August 22nd 2019This method presents the reversed-phase retention and detection of 7 polar Neonicotinoids and 4 deuterated-internal standards by LC-MS/MS and with a Kinetex 2.6 Biphenyl HPLC column. The Kinetex Biphenyl phase demonstrated significant polar compound retention and selectivity.
Effectiveness of Polar Stationary Phase Modification on Peak Shape for Basic Compounds
August 22nd 2019Described in this application note are the details for an investigation of basic compound peak shape across four different alkyl C18 stationary phases that are all based upon the same core-shell HPLC/UHPLC particle morphology.
Comparison of the Reversed-Phase Retention of Six Extremely Polar Artificial Sweeteners
August 22nd 2019In this application, is the investigation into the relative selectivity of four different C18 stationary phases when applied to the reversed phase retention of six extremely polar artificial sweeteners.
mAb Aggregation Using a Cary 60 UV-vis Spectrophotometer
June 22nd 2017The advantage of UV spectroscopy as an analytical method to detect protein aggregation is that it is non-destructive and uses low sample volumes, minimal sample preparation requirement and easy sample analysis. A quick QC method to indicate the presence of aggregation uses an Agilent Cary 60 UV-Vis spectrophotometer to identify aggregation of mAb solutions resulting from different stress conditions.
