Tips & Tricks: GPC/SEC Copolymer Analysis in GPC/SEC 1: Compositional Analysis by GPC/SEC with Multiple Detection
GPC/SEC analysis reveals a multitude of molar mass information about unknown samples that are chemically simple or homogenous.
GPC/SEC analysis reveals a multitude of molar mass information about unknown samples that are chemically simple or homogenous.
However, results for copolymers may be compromised by the fact that fractions with different composition and different molar mass may co-elute.
There are different methods on how to characterize such samples:
• chemical composition analysis studied in GPC/SEC mode by concentration detectors (this issue)
• chemical heterogeneity analysis investigated by HPLC based on retention differences of samples with different composition (upcoming issue)
• comprehensive 2D analysis that allows samples to be separated by composition and molar mass simultaneously (upcoming issue)
This issue will discuss the chemical composition analysis with multiple detection, while the latter two will be discussed in the following two GPC/SEC: Tips & Tricks issues.
The Complexity of Oligonucleotide Separations
January 9th 2025Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.
Metabolomics Analysis of Low Birth-Weight Infants Using UHPLC-MS/MS Following Lipid Emulsion
January 10th 2025A recent study aimed to directly compare the changes in serum metabolites among very low birth-rate (VLBW) infants following the administration of the soybean oil-based lipid emulsion and soybean oil, medium-chain triglycerides, olive oil, and fish oil (SMOF) lipid emulsion using untargeted metabolomics techniques.
Analyzing New Drug Modalities: An ISC 2024 Interview with Kelly Zhang
January 10th 2025At ISC 2024 in Liverpool, United Kingdom, LCGC International interviewed Kelly Zhang of Genentech about her work analyzing new drug modalities, such as mRNA, oligonucleotides, peptides, and cell and gene therapies.
