Ruggedness of YMC Cyano-High Strength Stationary Phase Under Acidic and Basic Extremes
The Application Notebook
Silica-based cyano stationary phases have historically exhibited poor stability and short column lifetime at pH extremes. This application note details an investigation of the stability of YMC's Cyano-HG (high strength) 10 ?m silica-based stationary phase, which was performed at a customer's request.
Silica-based cyano stationary phases have historically exhibited poor stability and short column lifetime at pH extremes. This application note details an investigation of the stability of YMC's Cyano-HG (high strength) 10 μm silica-based stationary phase, which was performed at a customer's request. The columns were stressed under acidic (0.1% trifluoroacetic acid) and basic (10 mM ammonium bicarbonate, pH = 8.2) conditions, and evaluated for retention time, USP tailing, and USP plate count using two probes (naphthalene and methyl benzoate) and the following method:
An example chromatogram for the column performance test can be seen below:
The first column was run under the basic test conditions, exhibiting a modest loss in retention time for both analytes (0.087 min for methyl benzoate and 0.265 min for naphthalene). Tailing factor remained at 1.0 for both analytes and plate count was also very stable for both over the entire 50 h span.
Figure 1: Cyano-HG stability - basic conditions.
The second column was tested in the same manner except under the acidic conditions. This column exhibited essentially no loss in retention time (0.010 min) for methyl benzoate and only a slight decrease in retention time for naphthalene (0.065 min). Tailing factor remained unchanged (1.2 for methyl benzoate and 1.1 for naphthalene) and plate count was very stable under these conditions, similar to what was seen during the basic stress runs.
Figure 2: Cyano-HG stability - acidic conditions.
Overall, 10 μm YMC-Pack Cyano-HG stationary phase proved to be very rugged under both stress conditions and is suitable for use in preparative separations when used under the test conditions.
YMC America, Inc.
941 Marcon Blvd., Suite 201, Allentown, PA 18109
tel. (610) 266-8650
Website: www.ymcamerica.com
Infographic: Be confidently audit ready, at any time and reduce failures in pharma QC testing
November 20th 2024Discover how you can simplify the audit preparation process with data integrity dashboards that provide transparency to key actions, and seamlessly track long-term trends and patterns, helping to prevent system suitability failures before they occur with waters_connect Data Intelligence software.
6PPD-Quinone Reference Materials
November 19th 2024Ensure environmental and consumer health with our standards for 6PPD-quinone testing. 6PPD-quinone has been detected in the environment and has shown toxicity to aquatic life. Chiron, by Zeptometrix® offers reference standards suitable for Draft EPA Method 1634.
Current and Future Advancements in PFAS Research
November 19th 2024This white paper explores the health risks, environmental impacts, and detection technologies associated with PFAS, along with the latest advancements in PFAS research. It also provides an overview of the regulatory landscape and emphasizes the crucial role of companies like ZeptoMetrix® in supplying PFAS reference materials, which are essential for ensuring the accuracy of testing. Lastly, the paper outlines key areas for future PFAS research.
Microplastics Reference Materials
November 19th 2024The World’s First Microplastics Reference Materials. Our scientists have focused on these emerging global threats, and are excited to share Chiron MicroPrefs®, the first commercial microplastic reference material. The MicroPref® portfolio is designed to detect the six most abundant plastics in the environment and is available in a novel, easy-to-use tablet formulation. Be among the first labs to join in the fight against microplastic pollution by exploring the NEW line of Chiron MicroPrefs® microplastic standards.
