Fast and Robust Analysis of Drugs of Abuse Using Direct Exposure Probe on the Pegasus HT
The Application Notebook
Direct exposure probe (DEP) coupled to the Pegasus HT, time-of-flight mass spectrometer, was used for the identification of synthetic cannabinoids in MTN-series compounds.
Direct exposure probe (DEP) coupled to the Pegasus HT, time-of-flight mass spectrometer, was used for the identification of synthetic cannabinoids in MTN-series compounds.
Crime laboratories are responsible for the timely identification of seized drugs used as evidence in criminal investigations. The objective was to identify drugs of abuse using DEP instead of traditional LC or GC for time-saving and ease-of-use.
Experimental Conditions
Results
The MTN-series of compounds are allegedly blends of JWH-series compounds, but have not been studied as extensively. Very rapidly, it was determined that MTN-767 is most likely a blend of JWH-018 and JWH-081 (Figure 1). True Signal Deconvolution® produced two mass spectra that were compared to the NIST, Wiley, and Cayman library databases and yielded hits with similarity scores greater than 800 out of 1000. This result, as well as the analysis of other MTN compounds, confirmed that MTN-series compounds are blends of JWH compounds, and was accomplished within a few min.
Figure 1: DEP analysis was used for the fast and reliable identification of the unknown components in MTN-767, a recreational synthetic cannabinoid.
Advantages of DEP experiments on LECO's Pegasus HT:
1. Open source design - robust and low maintenance
2. Time-of-flight MS - no mass spectral skewing
3. True Signal Deconvolution® - identify more components
4. Probe installation does not interfere with GC or GC×GC
LECO Corporation
3000 Lakeview Avenue, St. Joseph, MI 49085
tel. (269) 985-5496, fax (269) 982-8977
Website: www.leco.com
The Benefits of Custom Bonded Silica
April 1st 2025Not all chromatography resins are created equal. Off-the-shelf chromatography resins might not always meet the rigorous purification requirements of biopharmaceutical manufacturing. Custom bonded silica from Grace can address a wide range of separation challenges, leading to real performance improvements. Discover more about the latest innovations in chromatography silica from Grace, including VYDAC® and DAVISIL®.
5 Things to Consider When Selecting a Chromatography Silica
April 1st 2025Particularly in the pharmaceutical industry, drug purity isn’t just a goal – it’s essential for achieving safety, stability and efficacy. However, purification is easier said than done, especially with challenging molecules like DNA and RNA “oligonucleotides,” due in large part to their diversity and the range of impurities that can be generated during production. Enter DAVISIL® chromatographic silica, with a wide range of pore diameters and particle sizes to meet your specific application, performance and sustainability requirements. Before you choose the chromatography resin for your next purification application, take a look at these 5 considerations.
Automating Protein Purification: Efficiency, Yield, and Reproducibility
March 27th 2025Recent advancements in automated protein purification stress the importance of efficiency, scalability, and yield consistency. This eBook compares different purification platforms, highlighting their impact on downstream applications and demonstrating how automation enhances throughput and process control.
MilliporeSigma: Ultrapure Water for Sensitive LC-MS Analysis of Pesticides
March 25th 2025The aim of the study was to illustrate the efficiency of Milli-Q® water purification systems in eliminating pesticides from tap water, thereby producing and delivering reliable and consistent-quality ultrapure water suitable for pesticides analysis
