Recent Developments in Column Technologies for the Analysis of Proteins and Peptides
Special Issues
Column developments in HPLC have benefited protein aqnd peptide separations in a number of ways. More silica based phases and column formats have become available, expanding the use of HPLC for the separation of proteins by different modes of chromatography as well as improving resolution and throughput. Hydrophobic interaction chromatography, immobilized metal affinity chromatography as well as ion Exchange and size exclusion are now practical analytical tools for proteins and peptides. New enrichment columns are benefiting proteomic research.
Best of the Week: Food Analysis, Chemical Migration in Plastic Bottles, STEM Researcher of the Year
December 20th 2024Top articles published this week include the launch of our “From Lab to Table” content series, a Q&A interview about using liquid chromatography–high-resolution mass spectrometry (LC–HRMS) to assess chemical hazards in plastic bottles, and a piece recognizing Brett Paull for being named Tasmanian STEM Researcher of the Year.
Using LC-MS/MS to Measure Testosterone in Dried Blood Spots
December 19th 2024Testosterone measurements are typically performed using serum or plasma, but this presents several logistical challenges, especially for sample collection, storage, and transport. In a recently published article, Yehudah Gruenstein of the University of Miami explored key insights gained from dried blood spot assay validation for testosterone measurement.
Determination of Pharmaceuticals by Capillary HPLC-MS/MS (Dec 2024)
December 19th 2024This application note demonstrates the use of a compact portable capillary liquid chromatograph, the Axcend Focus LC, coupled to an Agilent Ultivo triple quadrupole mass spectrometer for quantitative analysis of pharmaceutical drugs in model aqueous samples.
