Pickering Laboratories - Analysis of Mycotoxins in Hemp and Hemp- Containing Edible Products
Pickering Laboratories developed an easy and sensitive method to analyze aflatoxins B1, B2, G1, G2, and ochratoxin A in hemp and hemp-containing edible products. Mycotoxins are isolated using immunoaffinity clean-up columns and analyzed with fluorescence detection. To increase sensitivity of aflatoxins B1 and G1, an in-line photochemical reactor (UVETM) is installed before the detector. This method utilizes standard HPLC equipment and allows laboratories to easily determine these mycotoxins at low ppb levels.
Method
Isolation of aflatoxins B1, B2, G1, G2, and ochratoxin A
Blend 1 g of finely ground sample with extraction solution (10 mL of methanol/water 80:20) using a handheld homogenizer. Centrifuge for 10 min. Mix 2 mL of the extract with 10 mL of PBS buffer (containing 2% Tween 20). Clean the extracts using AflaOchra HPLC immunoaffinity column (Vicam).
Analytical Conditions
Analytical Column: MycotoxTM (Pickering), C18, 4.6 × 250 mm
HPLC eluent: sodium phosphate buffer (Cat #1700–1108), methanol, acetonitrile
Flow rate: 1 mL/min
Post-column photochemical reactor: UVE (Cat # 10519 (240V), Cat # 10742 (120 V) FLD: Excitation 36 nm, Emission 430 nm for Aflatoxins Excitation 333 nm, Emission 477 nm for Ochratoxin A.
Calibration
The 5-point calibration curves were built in the ranges of 0.325– 3.25 ppb for B1, 0.088–0.882 ppb for B2, 0.310–3.099 ppb for G1, 0.099–0.996 ppb for G2, and 1–10 ppb for ochratoxin A. Correlation coefficient was R2 >0.999 for all toxins.
Figure 1: Chromatogram of hemp pre-roll spiked with 6.5 ng/g of aflatoxin B1; 1.8 ng/g of aflatoxin B2; 6.1 ng/g of aflatoxin G1; 1.9 ng/g of aflatoxins G2; and 20.1 ng/g of ochratoxin A.
Figure 2: Chromatogram of hemp-containing chocolate spiked with 6.5 ng/g of aflatoxin B1; 1.8 ng/g of aflatoxin B2; 6.1 ng/g of aflatoxin G1; 1.9 ng/g of aflatoxins G2; and 20.1 ng/g of ochratoxin A.
Pickering Laboratories
1280 Speca Park Way, Mountain View, CA 04043
tel: (800) 654-3330, (650) 694-6700
Website: www.pickeringlabs.com
Using LC-MS/MS Analysis for PFAS Detection in Biological Samples and Serum
November 1st 2024Per- and polyfluoroalkyl substances (PFAS) are exceptionally versatile chemicals and have seemingly endless applications. Due to their persistent nature; prevalence in blood, food and environment; and emerging toxicity, there is a significant concern for their presence in the human body. Analysis is especially complicated due to background contamination in laboratory supplies and system. This paper will discuss sample preparation and LC-MS/MS analysis of an expanded panel of PFAS compounds in serum.
Robust Oligonucleotide Molecular Weight Confirmation via LC/MS
October 31st 2024Oligonucleotides—therapeutic agents for difficult-to-treat diseases—rely on precise molecular weight confirmation to ensure quality during development. Experts from Agilent Technologies explore the challenges of liquid chromatography-mass spectrometry (LC/MS) analysis in oligonucleotide synthesis, as well as strategies for accurate deconvolution, method development, and optimal equipment selection.
Development of a Fully Scalable High Efficiency 5um Solid-Core Particle to Support HPLC Workflows
October 30th 2024In this work the development of two key attributes of the 5um CORTECS Columns is examined. First column efficiency is compared across CORTECS and other solid-core 5 μm columns. Next scalability from sub-2 μm to 5 μm particles is examined between the CORTECS Column lines and competitive column lines. It was found that CORTECS columns have higher efficiency compared to other solid-core columns and that CORTECS particles are fully scalable.
