70-min Amino Acid Analysis of Physiological Samples
Pickering Laboratories specializes in the manufacturing of cation-exchange columns and eluants for amino acid analysis. Post-column derivatization with Ninhydrin offers unmatched selectivity and reproducibility of the analysis for most challenging matrixes.
Pickering Laboratories specializes in the manufacturing of cation-exchange columns and eluants for amino acid analysis. Post-column derivatization with Ninhydrin offers unmatched selectivity and reproducibility of the analysis for most challenging matrixes.
Table I: HPLC program
Pinnacle PCX post-column derivatization system allows you to combine eluant gradient capabilities of modern HPLC instruments with column temperature gradients. We introduce accelerated amino analysis method for physiological samples that utilizes temperature and eluant gradients.
Figure 1: List of amino acids: 1) Phosphoserine, 2) Taurine, 3) Phosphoethanolamine, 4) Urea, 5) Aspartic acid, 6) Hydroxyproline, 7) Threonine, 8) Serine, 9) Asparagine, 10) Glutamic acid, 11)Glutamine, 12) Sarcosine, 13) α-Aminoadipic acid, 14) Proline, 15) Glycine, 16) Alanine, 17) Citrulline, 18) α-Amino-n-butyric acid, 19) Valine, 20) Cystine, 21) Methionine, 22) Cystathionine, 23) Isoleucine, 24) Leucine, 25) Tyrosine, 26) Phenylalanine, 27) β-Alanine 28) β-Amino-i-butyric acid, 29) Homocystine, 30) γ-Aminobutyric acid, 31) Tryptophan, 32) Ethanolamine, 33) Ammonia, 34) Hydroxylysines, 35) Ornitine, 36) Lysine, 37) 1-Methylhistidine, 38) Histidine, 39) 3-Methylhistidine, 40) Anserine, 41) Carnosine, 42) Arginine.
Method
Analytical Conditions
Column: High-efficiency Lithium cation-exchange column (4.6 × 75 mm), 5 µm Catalog No. 0354675T
Flow Rate: 0.55 mL/min
Mobile Phase: 1700–1125, Li365, Li375, RG003
Table II: Column oven program
Post-column conditions
Post-column system: Pinnacle PCX
Reactor Volume: 0.5 mL
Temperature: 130 °C
Reagent: Trione
Flow Rate: 0.5 mL/min
Detection: UV-vis 570 nm for primary amino acids 440 nm for secondary amino acids
Run Time: 72 min
Equilibration Time: 15 min
Pickering Laboratories, Inc
1280 Space Park Way, Mountain View, CA 94043
tel. (800) 654-3330, (408) 694-6700
Website: www.pickeringlabs.com
SEC-MALS of Antibody Therapeutics—A Robust Method for In-Depth Sample Characterization
June 1st 2022Monoclonal antibodies (mAbs) are effective therapeutics for cancers, auto-immune diseases, viral infections, and other diseases. Recent developments in antibody therapeutics aim to add more specific binding regions (bi- and multi-specificity) to increase their effectiveness and/or to downsize the molecule to the specific binding regions (for example, scFv or Fab fragment) to achieve better penetration of the tissue. As the molecule gets more complex, the possible high and low molecular weight (H/LMW) impurities become more complex, too. In order to accurately analyze the various species, more advanced detection than ultraviolet (UV) is required to characterize a mAb sample.
