February 2022

A novel approach using hydrophilic-phase extraction (HPE) for the analysis of oligonucleotides in bioanalysis applications is described. The HPE method was proven to be a critical part of a fully quantitative HILIC–high-resolution mass spectrometry (HRMS) method for the RNA oligonucleotide and has potential for general application.

Kinetic plots can help us understand how different combinations of parameters will perform in relation the time needed to acquire a particular column efficiency—and thus resolution.

This month we discuss the consequences of the Golay theory, its relevance, and some alternatives.

Due to the enormous interest in a 2018 “Column Watch” article on the subject and the high level of research in the field, this article explores recent (2019–present) activity specifically towards the application of MOFs for liquid chromatography.

A balance printout is a fixed record, and is also called static data. But how static are static data when the weight is used in a chromatographic analysis? Also, have some regulatory data integrity guidance documents failed to comply with their own regulations?