Application Notes

This application note describes the molecular weight determination of different polymer samples under micro-GPC conditions with up to 80% less solvent consumption.

This applicationnote describesthe molecular weight determination of different polymer samplesunder micro-GPC conditions with up to 80% less solvent consumption.

The timsTOF fleX system bridges a current gap by providing MALDI Imaging and in-depth proteomics analysis in just one instrument. The instrument offers all benefits of a timsTOF Pro for time-efficient and sensitive proteomics, combined with a high-resolution MALDI source and stage. Using PASEF technology, it is possible to retrieve high protein ID rates with small sample amounts. Here we present the new SpatialOMx® workflow to identify distinct proteomic profiles for different tumor subpopulations in breast cancer as an example for this powerful approach.

For effective analytical practices, it is important to understand and control the binding of analytes to filters and pre-filters. This summary discusses the effect of sample filtration on analyte binding.

Biogen and Postnova present data on the analysis of adeno-associated viruses (AAV) using Asymmetrical Flow Field-Flow Fractionation coupled to Multi-Angle Light Scattering detection (AF4-MALS). AAV are promising gene therapy delivery vehicles, whose efficacy may be negatively affected by the presence of viral aggregates. Due to its gentle separation and broad applicable size range, AF4-MALS is able to characterize AAV and their aggregates with high resolution and precision, thereby overcoming the drawbacks that column-based chromatography techniques often face when dealing with samples larger than 50 nm in size.

For effective analytical practices, it is important to understand and control the binding of analytes to filters and pre-filters. This summary discusses the effect of sample filtration on analyte binding.

This technical overview presents sample purification under low and high pH conditions via two columns on one system, with automated column and solvent switching.

This application describes the purification of paracetamol from crude sample in early-stage drug discovery. Outstanding purity (>99%) and recovery (>95%) are achieved.

This technical overview presents an entire purification workflow using a 1290 Infinity II Autoscale Preparative LC/MSD System with Automated Purification Software.

This technical overview describes how to increase fraction capacity by clustering multiple fraction collectors. Smart software helps keep track of the fractions.

This technical overview describes the automated homogenization feature of the 1290 Infinity II Preparative Open-Bed Sampler/Collector.

This application describes the isolation of cannabinoids from a hemp oil extract. Analytical- and preparative-scale HPLC with MS detection is used for separation.

MALS with SEC improves the efficiencies of the analysis of bionanoparticles that are difficult to be differentiated from the impurities during the purification process. 

The performance of the Empore oil and grease solid-phase extraction disk under EPA Method 1664, while reducing the total volume of n-hexane by 40%, is demonstrated.