Application Notes: General
RAFA 2024 Companion: Hot Topics in Food Analysis
October 28th 2024In collaboration with the organizers of the Recent Advances in Food Analysis 2024 conference in Prague, Czech Republic, LCGC International has created a comprehensive e-book featuring cutting-edge topics in food analysis. This collection includes articles on the latest analytical and bioanalytical methods for ensuring food quality and safety, along with interviews with this year’s presenters. Readers will gain valuable insights into current trends, challenges, and innovative solutions in food and natural products analysis.
Inside the Laboratory: Measuring Molecular Tracers of Emerging Aerosol Sources
October 14th 2024Inside the Laboratory is a joint series with LCGC International and Spectroscopy, profiling analytical scientists and their research groups at universities all over the world. This series spotlights the current chromatographic and spectroscopic research their group is conducting, and the importance of their research in analytical chemistry and specific industries.
Top-down characterization of engineered Bcl-xL proteoforms
October 11th 2024Top-down fragmentation enables rapid characterization of phosphorylated proteins without extensive sample preparation and digestion. In this study, electron capture dissociation (ECD) was used to fragment proteoforms of the cell death-related protein, Bcl-xL. Using these methods, 85–90% sequence coverage was achieved for Bcl-xL proteoforms, allowing for effective localization of phosphorylation within minutes.
Antibody peptide mapping using the new Agilent ExD cell
October 11th 2024Enhanced antibody analysis using electron capture dissociation (ECD) allows for precise glycan localization in low-abundance glycopeptides. This study compares the fragmentation of trastuzumab tryptic digests using ECD and collision-induced dissociation (CID). While CID generates abundant glycan HexNAc ions at 204 m/z, ECD preserves the labile glycan group, enabling accurate site localization.
Identification of Amino Acid Isomers Using Electron Capture Dissociation
October 11th 2024Electron capture dissociation produces distinct fragments of amino acid side chains, enabling the identification of isomeric amino acids such as leucine and isoleucine. This application note demonstrates the isomer identification workflow for peptides and intact proteins using the new Agilent ExD cell and ExDViewer software for fragment analysis.
Trends, Best Practices, and Analytical Challenges in Chemical Characterization of Medical Devices
October 7th 2024Chemical characterization of medical devices, including drug-device combination products, is crucial for ensuring regulatory compliance and patient safety by identifying and quantifying chemicals that may interact with the human body. This paper explores current trends, best practices, and regulatory developments in extractables and leachables (E&L) testing for medical devices.
Pick Your Poison: Isolation of Paclitaxel (Oct 2024)
October 4th 2024The diterpenoid, paclitaxel, which was identified as a potent chemotherapy agent for breast and ovarian cancer originates from the Pacific Yew tree. The isolation of paclitaxel from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.
Separation of Fat-Soluble Vitamin K Congeners in Under 6 Minutes (Oct 2024)
October 4th 2024With the growing utility of the K vitamin series of molecules, a need appears relevant for both food labeling of constituents and for research based separation of the natural and synthetic compounds of vitamin K. The fast simple method developed by Hamilton Company using the PRP-C18 column allows for simple efficient identification of the whole vitamin series (K2, K3, and K4).