Tips&Tricks: GPC/SEC What is the Best Detector Sequence?
GPC/SEC (gel permeation chromatography/size exclusion chromatography) methods generally contain multiple detectors in order to gather more information about the molecular structure of macromolecules.1. Up until now, however, the practice and the impact of detector combination has not been discussed.
GPC/SEC (gel permeation chromatography/size exclusion chromatography) methods generally contain multiple detectors in order to gather more information about the molecular structure of macromolecules.1 Such detectors include: concentration detectors [refractive index (RI) and ultraviolet (UV) are the most commonly used]; molar mass sensitive detectors (light scattering, viscometers, mass spectrometers); and information-rich detectors [Fourier transform infrared (FTIR), and nuclear magnetic resonance (NMR)]. Over the years the information and results available from different detector combinations have been the subject of various Tips & Tricks articles. Up until now, however, the practice and the impact of detector combination has not been discussed.
Best of the Week: Food Analysis, Chemical Migration in Plastic Bottles, STEM Researcher of the Year
December 20th 2024Top articles published this week include the launch of our “From Lab to Table” content series, a Q&A interview about using liquid chromatography–high-resolution mass spectrometry (LC–HRMS) to assess chemical hazards in plastic bottles, and a piece recognizing Brett Paull for being named Tasmanian STEM Researcher of the Year.
Using LC-MS/MS to Measure Testosterone in Dried Blood Spots
December 19th 2024Testosterone measurements are typically performed using serum or plasma, but this presents several logistical challenges, especially for sample collection, storage, and transport. In a recently published article, Yehudah Gruenstein of the University of Miami explored key insights gained from dried blood spot assay validation for testosterone measurement.
Determination of Pharmaceuticals by Capillary HPLC-MS/MS (Dec 2024)
December 19th 2024This application note demonstrates the use of a compact portable capillary liquid chromatograph, the Axcend Focus LC, coupled to an Agilent Ultivo triple quadrupole mass spectrometer for quantitative analysis of pharmaceutical drugs in model aqueous samples.
