Supplementary Appendix 1 to A Practical Approach to Modelling of Reversed-Phase Liquid Chromatographic Separations: Advantages, Principles, and Possible Pitfalls
LCGC Europe
This information is supplementary to the article “A Practical Approach to Modelling of Reversed-Phase Liquid Chromatographic Separations: Advantages, Principles, and Possible Pitfalls” that was published in the March 2018 LCGC Europe issue.
Determination of dwell volume
- Replace the column with a 100 cm x 0.1 mm capillary to generate enough pressure to ensure proper function of check valves.
- Use water as solvent A and 10 mg/L of uracil in water as solvent B.
- Set the flow rate to the flow that should be modelled.
- Programme a 1 µL injection of water to ensure that the auto-sampler is in the flow path and contributes to the dwell volume.
- Run a gradient with a 5-min isocratic hold followed by a 10-min linear gradient with the same slope as the average gradient used for collection of the retention modelling data, for example 10%B at 0 min, from 10 to 20%B between 5 and 15 min, from 20 to 10%B between 25 and 25.1 min.
- UV detection at 259 nm at 20 Hz to ensure a well-defined curve.
- The dwell volume is defined using the gradient start and stop time and time point for 50% of maximal signal as described in Figure 2(a).
- In order to evaluate the linearity (Figure 2) an extra run is made where the range is extended to 0–100%B. An extra gradient is necessary since flow rate and gradient range influence the determination of dwell volume.
AI and GenAI Applications to Help Optimize Purification and Yield of Antibodies From Plasma
October 31st 2024Deriving antibodies from plasma products involves several steps, typically starting from the collection of plasma and ending with the purification of the desired antibodies. These are: plasma collection; plasma pooling; fractionation; antibody purification; concentration and formulation; quality control; and packaging and storage. This process results in a purified antibody product that can be used for therapeutic purposes, diagnostic tests, or research. Each step is critical to ensure the safety, efficacy, and quality of the final product. Applications of AI/GenAI in many of these steps can significantly help in the optimization of purification and yield of the desired antibodies. Some specific use-cases are: selecting and optimizing plasma units for optimized plasma pooling; GenAI solution for enterprise search on internal knowledge portal; analysing and optimizing production batch profitability, inventory, yields; monitoring production batch key performance indicators for outlier identification; monitoring production equipment to predict maintenance events; and reducing quality control laboratory testing turnaround time.
2024 EAS Awardees Showcase Innovative Research in Analytical Science
November 20th 2024Scientists from the Massachusetts Institute of Technology, the University of Washington, and other leading institutions took the stage at the Eastern Analytical Symposium to accept awards and share insights into their research.
