Supplement analysis
Methylhexaneamine (MHA) has been in the news recently and it continues to be debated whether it is actually a constituent of geranium.
Methylhexaneamine (MHA) has been in the news recently and it continues to be debated whether it is actually a constituent of geranium. In a comprehensive analysis, a team of scientists has presented evidence that it is not present in the plant in detectable amounts.1
MHA has been marketed as a dietary supplement based on the argument that it is a constituent of geranium (Pelargonium graveolens) leaves, stems, roots or oil. The team developed two analytical methods for the analysis of MHA in P. graveolens using gas chromatography–mass spectrometry (GC–MS) and liquid chromatography–tandem mass spectrometry (LC–MS–MS). The results were further confirmed using liquid chromatography–high-resolution mass spectrometry. Twenty commercial volatile oils, three authenticated volatile oils and authenticated P. graveolens leaves and stems were analysed. In addition, three dietary supplements containing MHA which claimed P. graveolens as the source were analysed for their MHA content.
The results obtained demonstrated that none of the authenticated P. graveolens essential oils or plant material, nor the commercial volatile oils of contained MHA at detectable levels (limit of detection = 10 ppb). In contrast, the dietary supplements that contained MHA as one of their ingredients contained very large amounts of MHA. These amounts are not compatible with the use of reasonable amounts of P. graveolens extract or concentrate. The team ultimately concluded that this must be because the MHA was of a synthetic origin.
Mahmoud A. ElSohly et al., Journal of Analytical Toxicology doi: 10.1093/jat/bks055 (2012).
This story originally appeared in The Column. Click here to view that issue.
The Next Frontier for Mass Spectrometry: Maximizing Ion Utilization
January 20th 2025In this podcast, Daniel DeBord, CTO of MOBILion Systems, describes a new high resolution mass spectrometry approach that promises to increase speed and sensitivity in omics applications. MOBILion recently introduced the PAMAF mode of operation, which stands for parallel accumulation with mobility aligned fragmentation. It substantially increases the fraction of ions used for mass spectrometry analysis by replacing the functionality of the quadrupole with high resolution ion mobility. Listen to learn more about this exciting new development.
Liquid Chromatography to Analyze Vitamin D Proteins in Psoriasis Patients
January 21st 2025Can a protein involved in delivering Vitamin D to target tissues have an altered serum profile in psoriasis patients with cardiovascular disease? Researchers used liquid chromatography (LC) to help find out.
The Complexity of Oligonucleotide Separations
January 9th 2025Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.
A Guide To Finding the Ideal Syringe and Needle
January 20th 2025Hamilton has produced a series of reference guides to assist science professionals in finding the best-suited products and configurations for their applications. The Syringe and Needle Reference Guide provides detailed information on Hamilton Company’s full portfolio of syringes and needles. Everything from cleaning and preventative maintenance to individual part numbers are available for review. It also includes selection charts to help you choose between syringe terminations like cemented needles and luer tips.
