Separation Technology in Drug Discovery

A Monday session at the Eastern Analytical Symposium, sponsored by the Chinese American Chromatography Association, explored key challenges and solutions for achieving more sensitive oligonucleotide analysis.

The method of standard additions (MoSA) enables quantification of volatile impurities in condensed-phase samples, such as emulsions, for which matrix-matched calibration standards are required. The technique is, however, expensive because it requires multiple analyses of each sample.

This article will discuss an ion-pair reversed-phase liquid chromatography mass spectrometry (IP-RPLC–MS) workflow for the characterization of three messenger RNA (mRNA) key critical quality attributes: 5’ Cap, ORF, and poly(A) tail.

Pharmaceutical excipients, such as polyethylene glycol-based polymers, must be tested for the presence of ethylene oxide (EtO) and 1,4-dioxane as part of a safety assessment, according to USP Chapter <228>.

Mithibai College of Arts scientists recently used liquid chromatography-tandem mass spectrometry (LC–MS/MS) for detecting nitrosamines in blood pressure medication.

Scientists recently developed new GC/LC-Q-TOF/MS-based method for determining pesticides in Angelica sinensis, a traditional Chinese medicine.