Separation of a Mix of Acidic, Basic, and Neutral Compounds at High pH Conditions
The unique surface chemistry of the Flare diamond core-shell column combines ionic and hydrophobic separation mechanisms to effectively retain a variety of chemical species in a single run.
The unique surface chemistry of the Flare diamond core-shell column combines ionic and hydrophobic separation mechanisms to effectively retain a variety of chemical species in a single run.
HPLC Conditions
Column Name: Flare C18 Mixed-Mode Column
Dimensions: 4.6 × 33 mm, 3.6 µm, 180 Å
HPLC System: Waters® 1525 Binary Pump
Injection Volume: 5 µL
Detection: UV at 254 nm
Flow Rate: 1.0 mL/min
Separation Mode: Isocratic
Mobile Phase: A: 10 mM phosphate buffer, pH 12; B: acetonitrile A/B (70:30)
Temperature: 35 °C
Analytes:
Conclusions
1) Fast separation at high pH
2) Separation possible with 100% aqueous mobile phase
3) Straightforward method transfer to any system
Diamond Analytics
11260 South 1600 West, Orem, UT 84058
tel. (801) 235-9001, fax (801) 235-9141
Website: diamond-analytics.com
Determination of Pharmaceuticals by Capillary HPLC-MS/MS (Dec 2024)
December 19th 2024This application note demonstrates the use of a compact portable capillary liquid chromatograph, the Axcend Focus LC, coupled to an Agilent Ultivo triple quadrupole mass spectrometer for quantitative analysis of pharmaceutical drugs in model aqueous samples.
Axcend Focus LC® Cartridge Options
December 19th 2024Welcome to our comprehensive selection of High-Performance Liquid Chromatography (HPLC) cartridges. Designed for precision and reliability, our cartridges cater to diverse analytical needs, ensuring optimal performance in your laboratory processes.
