Nanotechnology Sensor Detects Melanoma
Scientists from the Monell Chemical Senses Center (Philadelphia, USA) have developed a nanotechnology sensor for the detection of melanoma.1
Scientists from the Monell Chemical Senses Center (Philadelphia, USA) have developed a nanotechnology sensor for the detection of melanoma.1 Melanoma arises when melanocytes, pigment-producing cells that give skin its colour, transform to become tumours. The disease is estimated to be responsible for 75% of skin cancer deaths, according to a press release from the center. Detection is largely dependent on self‑examination, and subsequent visual diagnosis by a clinician.
A need for a non-invasive method for the detection of melanoma led the group to consider the volatile organic compounds (VOCs) released by the skin. Based on previous studies, they hypothesized that the VOC profile of melanoma and melanocytes could differ.
Solid-phase micro-extraction (SPME) was performed followed by gas chromatography–mass spectrometry (GC–MS) to determine variations between the VOC profiles of melanocytes and melanoma. Levels of isoamyl alcohol were found to be higher in melanoma cells than melanocytes, and isolvaleric acid lower in melanoma cells. Melanoma cells produced dimethyldi- and trisulphide compounds, not detected from non-melanoma cells.
Recognizing the need for a portable method that could be transferred into a clinical setting, the scientists developed a single-stranded DNA-coated nanotube (DNACNT) sensor to examine VOCs from melanoma and normal cells. The sensor consisted of nano-sized carbon tubes coated with DNA that could be bioengineered to recognize different VOCs associated with other diseases.
The authors concluded that the monitoring of melanoma VOCs has potential in screening methods. A.T. Charlie Johnson (University of Pennsylvania), who led the development of the sensor, commented: “We are excited to see that the DNA-carbon nanotube vapour sensor concept has potential for use as a diagnostic. Our plan is to move forward with research into skin cancer and other diseases.”
Reference
1. Jae Kwak et al, Journal of Chromatography B931, 90–96 (2013).
This story originally appeared in The Column. Click here to view that issue.
The Next Frontier for Mass Spectrometry: Maximizing Ion Utilization
January 20th 2025In this podcast, Daniel DeBord, CTO of MOBILion Systems, describes a new high resolution mass spectrometry approach that promises to increase speed and sensitivity in omics applications. MOBILion recently introduced the PAMAF mode of operation, which stands for parallel accumulation with mobility aligned fragmentation. It substantially increases the fraction of ions used for mass spectrometry analysis by replacing the functionality of the quadrupole with high resolution ion mobility. Listen to learn more about this exciting new development.
Liquid Chromatography to Analyze Vitamin D Proteins in Psoriasis Patients
January 21st 2025Can a protein involved in delivering Vitamin D to target tissues have an altered serum profile in psoriasis patients with cardiovascular disease? Researchers used liquid chromatography (LC) to help find out.
The Complexity of Oligonucleotide Separations
January 9th 2025Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.
A Guide To Finding the Ideal Syringe and Needle
January 20th 2025Hamilton has produced a series of reference guides to assist science professionals in finding the best-suited products and configurations for their applications. The Syringe and Needle Reference Guide provides detailed information on Hamilton Company’s full portfolio of syringes and needles. Everything from cleaning and preventative maintenance to individual part numbers are available for review. It also includes selection charts to help you choose between syringe terminations like cemented needles and luer tips.
