Direct Analysis of Complex Mixtures Using U-HPLC and High Resolution Mass Spectrometry
This article will demonstrate the feasibility of analysing complex mixtures without any prior sample preparation by making use of the high resolving power of both UHPLC and mass spectrometry.
Red wine is a very complex mixture and a rich source of beneficial anti-oxidants. Identification and quantification of these natural products is challenging. Ultra-high pressure liquid chromatography (U-HPLC) coupled to a mass spectrometer is used for the analysis of French red wine, enabling simultaneous detection and relative quantification of the wine’s antioxidant constituents. Using this technique, the phenolic compounds (such as quercetin) responsible for most of the health benefits associated with the consumption of red wine can be identified and their variable content across two different harvest years can be observed. Direct wine analysis approach is then applied to monitor the progressive changes in red wine after its exposure to air. The successful use of this approach has meant metabolomic analysis has become a rapidly growing area of interest in nutrition and health research. This article will demonstrate the feasibility of analysing complex mixtures without any prior sample preparation by making use of the high resolving power of both U-HPLC and mass spectrometry
LCGC’s Year in Review: Highlights in Liquid Chromatography
December 20th 2024This collection of technical articles, interviews, and news pieces delves into the latest innovations in LC methods, including advance in high performance liquid chromatography (HPLC), ultrahigh-pressure liquid chromatography (UHPLC), liquid chromatography–mass spectrometry (LC–MS), and multidimensional LC.
Analytical Challenges in Measuring Migration from Food Contact Materials
November 2nd 2015Food contact materials contain low molecular weight additives and processing aids which can migrate into foods leading to trace levels of contamination. Food safety is ensured through regulations, comprising compositional controls and migration limits, which present a significant analytical challenge to the food industry to ensure compliance and demonstrate due diligence. Of the various analytical approaches, LC-MS/MS has proved to be an essential tool in monitoring migration of target compounds into foods, and more sophisticated approaches such as LC-high resolution MS (Orbitrap) are being increasingly used for untargeted analysis to monitor non-intentionally added substances. This podcast will provide an overview to this area, illustrated with various applications showing current approaches being employed.
Using Chromatography to Study Microplastics in Food: An Interview with Jose Bernal
December 16th 2024LCGC International sat down with Jose Bernal to discuss his latest research in using pyrolysis gas chromatography–mass spectrometry (Py-GC–MS) and other chromatographic techniques in studying microplastics in food analysis.
The Use of SPME and GC×GC in Food Analysis: An Interview with Giorgia Purcaro
December 16th 2024LCGC International sat down with Giorgia Purcaro of the University of Liege to discuss the impact that solid-phase microextraction (SPME) and comprehensive multidimensional gas chromatography (GC×GC) is having on food analysis.
