Andrea Angelo P. Tripodi joined Agilent Technologies as an applications chemist in September 2019 after completing his MSCA PhD in organic chemistry, which was specifically focused on the development of peptide drug‑conjugates, at ELTE University in Budapest (Hungary). He develops LC applications for biomolecule analysis and purification using a wide variety of approaches, including reversed-phase, size exclusion, ion exchange, hydrophilic interaction liquid chromatography (HILIC), and hydrophobic interaction chromatography (HIC).
Systematic Evaluation of HILIC Stationary Phases for MS Characterization of Oligonucleotides
Hydrophilic interaction chromatography–mass spectrometry (HILIC-MS) offers a flexible and efficient alternative to ion-pairing reversed-phase liquid chromatography (IP-RPLC) for oligonucleotide analysis, with column selectivity and mobile phase pH being key factors in optimizing retention and detection.