The Column-11-06-2019
Diagnosing Cancer Using Cerumen and HSGC–MS
November 6th 2019The Column spoke to Nelson Roberto Antoniosi Filho, a professor at the Chemistry Institute of the Federal University of Goiás (UFG), in Goiânia, Brazil, about his development of a gas chromatography–mass spectrometry (GC–MS) method for cancer diagnosis using cerumen.
Challenging Allergen Uncertainty Using LC–MS/MS
November 6th 2019Researchers attempting to overcome complex matrix interference in commercial food analysis have developed a targeted LC–MS/MS method and open source analysis software capable of identifying allergens in complex food matrices.
High Sensitivity Native Mass Spectrometry with Cation Exchange Chromatography
November 6th 2019The utility of native high-resolution mass spectrometry (HRMS) in intact protein characterization is rapidly growing because of advances in both ion-exchange chromatography (IEC) as well as MS-compatible buffer systems. MS is a critical component of biotherapeutic characterization, but its combination with traditional chromatographic separations, such as size-exclusion chromatography (SEC) and IEC, has been slow because of the predominant use of high salt mobile phases, which are incompatible with MS. Recently reported methods using cation-exchange chromatography (CEX) with volatile buffer systems for pH gradient elution has given researchers the ability to use these chromatographic techniques with MS detection. In this article a robust, MS-compatible buffer system for high sensitivity IEC with pH gradient elution for charge variant analysis of intact monoclonal antibodies (mAbs) is described.
