Phenomenex, Inc.

The development of an automated online SPE–LC–MS-MS method for the analysis of 25-OH-vitamin D2, 25-OH-vitamin D3, and 3-Epi-25-OH-vitamin D3 from human serum.

Enhancing the extraction of vitamin A and E in serum using simplified liquid extraction (SLE) significantly improves the LC–MS-MS detection of target compounds without an overly complex method or sacrificing robustness.

The development of an automated online SPE–LC–MS-MS method for the analysis of 25-OH-vitamin D2, 25-OH-vitamin D3, and 3-Epi-25-OH-vitamin D3 from human serum.

A full ESI+/ESI- 52 drug panel was performed in 7 min and 30 s, including re-equilibration (44 in ESI+ and 8 in ESI-). This rapid analysis showed great resolution and no loss in sensitivity compared to slower methods.

A full ESI+/ESI- 52 drug panel was performed in 7 min and 30 s, including re-equilibration (44 in ESI+ and 8 in ESI-). This rapid analysis showed great resolution and no loss in sensitivity compared to slower methods.

Enhancing the extraction of vitamin A and E in serum using simplified liquid extraction (SLE) significantly improves the LC–MS-MS detection of target compounds without an overly complex method or sacrificing robustness.

A simple automated extraction method using a Tecan Freedom EVO® 100 liquid handler and Phenomenex’s Novum simplified liquid extraction (SLE) 96-well plate. Analysis of isomeric cortisone and prednisolone by UHPLC by Kinetex 2.6 μm, 50 x 3 mm core-shell Biphenyl column was used to successfully separate these two compounds and their isomers.

A simple automated extraction method using a Tecan Freedom EVO® 100 liquid handler and Phenomenex’s Novum simplified liquid extraction (SLE) 96-well plate. Analysis of isomeric cortisone and prednisolone by UHPLC by Kinetex 2.6 μm, 50 x 3 mm core-shell Biphenyl column was used to successfully separate these two compounds and their isomers.

Using a single LC–MS-MS application, 12 steroids were separated. However, three separate panels are also run as an alternative method.

This application note describes the GC¬¬–MS analysis of 23 drugs of abuse from various compound classes such as amphetamines, benzodiazepines, cannabinoids, and other illicit drugs.

A simple sample preparation procedure and a rapid, sensitive, specific UHPLC–MS-MS method has been developed for quantifying several antidepressants and metabolites.

Using a single LC–MS-MS application, 12 steroids were separated. However, three separate panels are also run as an alternative method.

A simple sample preparation procedure and a rapid, sensitive, specific UHPLC–MS-MS method has been developed for quantifying several antidepressants and metabolites.

This application note describes the GC¬¬–MS analysis of 23 drugs of abuse from various compound classes such as amphetamines, benzodiazepines, cannabinoids, and other illicit drugs.

The collection of oral fluid is gaining popularity due to the fact that it is not intrusive and easy to use. In this study we look at four different devices, and compare the results obtained for drugs varying in terms of hydrophobicity and their acidity/basicity.

The collection of oral fluid is gaining popularity due to the fact that it is not intrusive and easy to use. In this study we look at four different devices, and compare the results obtained for drugs varying in terms of hydrophobicity and their acidity/basicity.

Using a targeted solid phase extraction (SPE) method and GC–ECD analysis, chlorinated pesticides are extracted from poultry fat resulting in a method that decreases the amount of labor and reagents when compared to the USDA-FSIS CHC2 method.

Using a targeted solid phase extraction (SPE) method and GC–ECD analysis, chlorinated pesticides are extracted from poultry fat resulting in a method that decreases the amount of labor and reagents when compared to the USDA-FSIS CHC2 method.

EPA Method 625 is greatly simplified by using solid phase extraction (SPE) as an alternative to traditional liquid-liquid extraction (LLE). Using this SPE technique, paired with a sensitive GC–MS analysis, increases throughput and data quality, while decreasing manual labor and solvent usage.

Improving and optimizing EPA Method 593 using solid phase extraction (SPE) enhances sensitivity and maximizes efficiency while screening by LC–MS-MS for different female birth control hormones present in drinking and waste water.

EPA Method 625 is greatly simplified by using solid phase extraction (SPE) as an alternative to traditional liquid-liquid extraction (LLE). Using this SPE technique, paired with a sensitive GC–MS analysis, increases throughput and data quality, while decreasing manual labor and solvent usage.

Phospholipid removal proves to yield superior results as compared to a traditional protein precipitation step when analyzing a complex plasma matrix via LC–MS-MS. The results displayed a decrease in ion suppression, increased analyte sensitivity, and an improvement in column lifetime.

Enhancing the extraction of vitamins A and E in serum using simplified liquid extraction (SLE) significantly improves the LC–MS-MS detection of target compounds, all while preserving a simple and robust method.

Phospholipid removal proves to yield superior results as compared to a traditional protein precipitation step when analyzing a complex plasma matrix via LC–MS-MS. The results displayed a decrease in ion suppression, increased analyte sensitivity, and an improvement in column lifetime.

Enhancing the extraction of vitamins A and E in serum using simplified liquid extraction (SLE) significantly improves the LC–MS-MS detection of target compounds, all while preserving a simple and robust method.

Using oral fluid as the matrix, a viable and simple solid phase extraction method for a wide range of drugs is developed. Several oral fluid collection devices were evaluated to determine the effectiveness of the cleanup procedure, ultimately confirming that the method is both robust and widely applicable.

Improving and optimizing EPA Method 593 using solid phase extraction (SPE) enhances sensitivity and maximizes efficiency while screening by LC–MS-MS for different female birth control hormones present in drinking and waste water.

Using oral fluid as the matrix, a viable and simple solid phase extraction method for a wide range of drugs is developed. Several oral fluid collection devices were evaluated to determine the effectiveness of the cleanup procedure, ultimately confirming that the method is both robust and widely applicable.

An ultra-sensitive sample preparation technique using solid phase extraction (SPE) microelution 96-well plates effectively concentrates peptides and allows for cleanup of small volume.

A suite of unknown drug compounds from whole blood are analyzed by LC–MS-MS, requiring the need for an effective yet nonspecific cleanup technique. The resulting method demonstrates a simple and fast sample preparation procedure that is suitable for screening many compounds.