Charles Durant

Cytosine (chemical name 4-amino-2-hydroxypyrimidine) is a pyrimidine derivative with a hetereocyclic aromatic ring and two substituents (amine and keto groups) attached and is a polar compound of significant biological and pharmaceutical interest. In response to the intended use of bulk cytosine as a raw material in pharmaceutical manufacturing, a method for the determination of the purity of cytosine was developed.

The authors meet the need for a method for the determination of cytosine purity by developing a hydrophilic interaction chromatography (HILIC) method with demonstrated advantages in comparison to other separation techniques.