Application Notes: General

A Hamilton PRP-C18 (5 μm) HPLC column was used for purifying three oligonucleotides. Analysis of a 21mer, 22mer, and 23mer of similar structure were evaluated.

Sulfide compounds found in garlic oils are used in therapeutics and prophylaxis of certain cancers, and are prevalent in cell health.

Identification and quantification of the Raffinose Family of Oligosaccharides allows a more thorough understanding of an individual’s gut health.

Garlic has been found to exhibit a multitude of proliferative health benefits in the realm of cardiac events.

Isolation of capsaicin for medicinal uses is easily achieved with the PRP-C18 RP-HPLC resin.

Excellent separation and chemical stability in oligonucleotide isolation is observed. The C18 column provides loading capacities and reliability needed for repeat use.

Identification of the five most common saccharides found in packaged foods, lactose, maltose, sucrose, fructose, and glucose, is easily resolved on the RCX-30 column.

GRPC peptides are found in the entire body. Isolation of seven classic GRPC peptides initiating in the GI tract could implicate disease.

This app note introduces the next generation of SIFT-MS that revolutionizes volatile impurities analysis workflows through unparalleled speed, performance stability, and reproducibility. Learn about how eliminates the hassles associated with chromatography and outpaces traditional methods in the analysis of challenging analytes such as formaldehyde in a PEG excipient.

This application note describes head-to-head comparison of GC-FID and SIFT-MS analyses of Class 2A and 2B residual solvents. SIFT-MS provides greater than 11-fold increase in sample throughput and significantly reduces the time taken to report quantitative results (over six times faster for a full calibration set).

Learn about the latest advancement in real-time, direct injection mass spectrometry (MS) that is built to solve the most difficult analytical challenges. Never miss a contamination event again.

The characteristic flexibility, stability, high throughput, and fast time to report analytical results of the Syft Tracer next-generation SIFT-MS instrument apply across multiple headspace approaches for diverse matrices. This application note briefly summarizes the use of (1) dissolution, (2) multiple headspace extraction (MHE), and (3) the method of standard additions, then provides a guide for identifying the appropriate headspace approach for various matrices.

The power of vacuum ultraviolet (VUV) light absorption can be useful in problematic moisture determination areas. A new detector for gas chromatography (GC) based on VUV absorption can potentially detect ultra-trace levels of moisture in acetonitrile.

Here we introduce LC/MS analysis methods using the Shodex™ HILICpak™ VC-50 2D column for various cationic neurotransmitters.

Complete and clean trypsin digestion can be achieved with an enzyme that provides a balance of low levels of missed cleavage, non-specific cleavage, and trypsin autolysis. RapiZyme, a next generation trypsin, offers efficiency and reproducibility.

The HILICpak™ VG-50 series columns are able to achieve high recovery rates of saccharides, organic acids, and amino acids.

Hydrophilic Interaction Liquid Chromatography (HILIC) can provide better separation for oligosaccharide analysis than Size Exclusion Chromatography (SEC) mode does.

This application demonstrated the effectiveness of Shodex™ HILICpak™ VG-50 4E with its HILIC mode for the analysis of various sugars found in Japanese sake.

Thermal desorption GC-MS is used to quantify indoor limonene concentrations from diffusers.

Mucopolysaccharidosis and oligosaccharidosis are LSDs caused by enzyme deficiencies. Early diagnosis is key using chromatographic separation and MS/MS identification.

Columns of the YMC-Pack Diol SEC series (120–300 Å), which have distinct pore sizes, were tested for their ability to separate DNA of various lengths (10–300mer).

Demonstration of the sensitivity gain for the Pierce peptide mix, which is achieved when using UHPLC columns of different i.d.s, as well as a micro-LC approach.

mRNA coupled to EGFP (enhanced green fluorescent protein) is analyzed using a widepore YMC-Accura Triart Bio C4 RP column as well as a non-porous BioPro IEX QF column.

A siRNA duplex and its single strands are separated using different YMC size exclusion columns to identify the most suitable SEC column.

Reversed-phase LC–MS analysis of two phospholipids—LPC (16:0) and PC (34:2)—using a bioinert coated column in comparison with a regular stainless steel one.

Different oligonucleotide mixtures were analyzed using the bioinert coated column YMC-Accura Triart Diol-HILIC in comparison to the conventional stainless steel column.

Four phosphopeptides are analyzed using a bioinert coated YMC-Accura Triart C18 column. The column provides high intensities, recoveries, and great peak shapes.