Click the title above to open the LCGC Europe May 2013 regular issue, Vol 26 No 5, in an interactive PDF format.
Exploring Metabolite Alteration from Age-Related Macular Degeneration with UHPLC-MS/MS
February 19th 2025Exploring the alterations in metabolites of wet age-related macular degeneration (wAMD), metabolomic analysis was performed on the aqueous humor of 30 patients with wAMD receiving anti-vascular endothelial growth factor (anti-VEGF) treatments and 20 controls, via ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS).
The Next Frontier for Mass Spectrometry: Maximizing Ion Utilization
January 20th 2025In this podcast, Daniel DeBord, CTO of MOBILion Systems, describes a new high resolution mass spectrometry approach that promises to increase speed and sensitivity in omics applications. MOBILion recently introduced the PAMAF mode of operation, which stands for parallel accumulation with mobility aligned fragmentation. It substantially increases the fraction of ions used for mass spectrometry analysis by replacing the functionality of the quadrupole with high resolution ion mobility. Listen to learn more about this exciting new development.
Direct Analysis of Class 2 Residual Solvents Using Molecular Rotational Resonance Spectroscopy
February 19th 2025Molecular rotational resonance (MRR) spectroscopy operates in the microwave region, detecting transitions between pure rotational energy levels. Combined with adiabatic cooling, MRR produces highly selective spectra unique to each molecule’s atomic structure. Even slight changes in atomic position or mass result in distinct spectral differences, allowing MRR to directly analyze complex mixtures, including isomers, without chromatographic separation. This article presents a continuous headspace-MRR method for analyzing Class 2 residual solvents, including low-volatility solvents from Class 2 Mixture C. By evaluating solvents with diverse boiling points, the study demonstrates that MRR meets ICH and USP standards for most Class 2 and Class 3 solvents and half of Class 1 solvents.