Tips & Tricks: GPC/SEC Is This Real? Unexpected Peak Shapes
GPC/SEC samples often show broad and/or multimodal peaks that are not baseline separated and look strange to users of other chromatographic techniques. However, although many weird looking peaks are possible in macromolecular characterization, some peaks are stranger than others and it is worth checking if the observed signal truly describes the sample.
GPC/SEC samples often show broad and/or multimodal peaks that are not baseline separated and look strange to users of other chromatographic techniques. However, although many weird looking peaks are possible in macromolecular characterization, some peaks are stranger than others and it is worth checking if the observed signal truly describes the sample. Therefore, it is good to know some tricks and hints that help identify measuring artefacts.
Analyzing Vitamin K1 Levels in Vegetables Eaten by Warfarin Patients Using HPLC UV–vis
April 9th 2025Research conducted by the Universitas Padjadjaran (Sumedang, Indonesia) focused on the measurement of vitamin K1 in various vegetables (specifically lettuce, cabbage, napa cabbage, and spinach) that were ingested by patients using warfarin. High performance liquid chromatography (HPLC) equipped with an ultraviolet detector set at 245 nm was used as the analytical technique.
Removing Double-Stranded RNA Impurities Using Chromatography
April 8th 2025Researchers from Agency for Science, Technology and Research in Singapore recently published a review article exploring how chromatography can be used to remove double-stranded RNA impurities during mRNA therapeutics production.
The Effect of Time and Tide On PFAS Concentrations in Estuaries
April 8th 2025Oliver Jones and Navneet Singh from RMIT University, Melbourne, Australia discuss a recent study they conducted to investigate the relationship between tidal cycles and PFAS concentrations in estuarine systems, and offer practical advice on the sample preparation and LC–MS/MS techniques they used to achieve the best results.
Assessing Safety of Medications During Pregnancy and Breastfeeding with LC–MS/MS
Published: April 8th 2025 | Updated: April 8th 2025Researchers conducted a study on medications used in psychiatry and neurology during the perinatal period, assessing how antiepileptic drugs (AEDs) affect placental functions, including transport mechanisms, nutrient transport, and trophoblast differentiation. Several quantitative methods, such as those for antianxiety and hypnotic drugs, were established to evaluate the safety of pharmacotherapy during breastfeeding using liquid chromatography-tandem mass spectrometry (LC–MS/MS).
