Tips & Tricks: GPC/SEC Method Development
The complexity of method development for GPC/SEC is often underestimated. Although only isocratic separation is applied, the development of a robust and stable method can be challenging. It is important that measures are taken to develop a suitable method that will deliver long-term reproducible results, especially when biomolecules, ions or functional groups are present.
The complexity of method development for GPC/SEC is often underestimated. Although only isocratic separation is applied, the development of a robust and stable method can be challenging. It is important that measures are taken to develop a suitable method that will deliver long‑term reproducible results, especially when biomolecules, ions or functional groups are present.
Analyzing Vitamin K1 Levels in Vegetables Eaten by Warfarin Patients Using HPLC UV–vis
April 9th 2025Research conducted by the Universitas Padjadjaran (Sumedang, Indonesia) focused on the measurement of vitamin K1 in various vegetables (specifically lettuce, cabbage, napa cabbage, and spinach) that were ingested by patients using warfarin. High performance liquid chromatography (HPLC) equipped with an ultraviolet detector set at 245 nm was used as the analytical technique.
Removing Double-Stranded RNA Impurities Using Chromatography
April 8th 2025Researchers from Agency for Science, Technology and Research in Singapore recently published a review article exploring how chromatography can be used to remove double-stranded RNA impurities during mRNA therapeutics production.
The Effect of Time and Tide On PFAS Concentrations in Estuaries
April 8th 2025Oliver Jones and Navneet Singh from RMIT University, Melbourne, Australia discuss a recent study they conducted to investigate the relationship between tidal cycles and PFAS concentrations in estuarine systems, and offer practical advice on the sample preparation and LC–MS/MS techniques they used to achieve the best results.
Assessing Safety of Medications During Pregnancy and Breastfeeding with LC–MS/MS
Published: April 8th 2025 | Updated: April 8th 2025Researchers conducted a study on medications used in psychiatry and neurology during the perinatal period, assessing how antiepileptic drugs (AEDs) affect placental functions, including transport mechanisms, nutrient transport, and trophoblast differentiation. Several quantitative methods, such as those for antianxiety and hypnotic drugs, were established to evaluate the safety of pharmacotherapy during breastfeeding using liquid chromatography-tandem mass spectrometry (LC–MS/MS).
