Tips & Tricks: GPC/SEC: Absolute or True Molar Masses for Macromolecules - Mass Spectrometry as a New Solution
Many GPC/SEC set-ups provide molar masses and molar mass distributions based on evaluation with a molar mass calibration curve created using molar mass reference standards.
GPC/SEC separates macromolecules based on their hydrodynamic volume and, therefore, allows an ensemble of chains with different lengths to be separated into small fractions. Primary information from GPC/SEC is the signal intensity at a defined elution volume. Molar mass distribution information can be obtained by assigning a molar mass to the elution volume.
Many GPC/SEC set-ups provide molar masses and molar mass distributions based on evaluation with a molar mass calibration curve created using molar mass reference standards. Here the elution volume of a reference material with known molar mass is measured.
With this easy-to-use method it is possible to obtain reproducible results, to compare samples and to establish powerful quality control methods with detailed sample information.
However, in case of chemically or structurally different samples and reference materials the obtained molar masses will be apparent molar masses, which can differ significantly from the true molar masses. Based on the molar mass range and the application different methods can be applied to overcome this limitation.
AI and GenAI Applications to Help Optimize Purification and Yield of Antibodies From Plasma
October 31st 2024Deriving antibodies from plasma products involves several steps, typically starting from the collection of plasma and ending with the purification of the desired antibodies. These are: plasma collection; plasma pooling; fractionation; antibody purification; concentration and formulation; quality control; and packaging and storage. This process results in a purified antibody product that can be used for therapeutic purposes, diagnostic tests, or research. Each step is critical to ensure the safety, efficacy, and quality of the final product. Applications of AI/GenAI in many of these steps can significantly help in the optimization of purification and yield of the desired antibodies. Some specific use-cases are: selecting and optimizing plasma units for optimized plasma pooling; GenAI solution for enterprise search on internal knowledge portal; analysing and optimizing production batch profitability, inventory, yields; monitoring production batch key performance indicators for outlier identification; monitoring production equipment to predict maintenance events; and reducing quality control laboratory testing turnaround time.
