Thin Layer Chromatography
Thin layer chromatography (TLC) is a planar chromatographic technique introduced in the 1950s as a fast, easy and inexpensive method for qualitative analysis.
Thin layer chromatography (TLC) is a planar chromatographic technique introduced in the 1950s as a fast, easy and inexpensive method for qualitative analysis. Just as with column chromatography, the chemistry and size of the particles (as well as the thickness of the layer) affects the speed and nature of the separations that are possible. After the sample is applied near one edge, the plate is developed by dipping the edge into solvent and allowing the separation to take place. Once the process nears completion, the plate is dried and various methods can be used to derive qualitative and quantitative measurements from the plate. These methods include densitometry, fluorometry, and even mass spectrometry.
2006 Thin Layer Chromatography Industrial Demand
The three main industrial applications for thin layer chromatography are in clinical, pharmaceutical and food testing. Combined, these three industries represent nearly 60% of the total demand for TLC. One of the most common specific types of clinical tests performed with TLC is for the presence of drugs of abuse. In the pharmaceutical industry, TLC is widely used in production and quality assurance applications. The presence or absence of substances can be determined qualitatively, while quantitative tests can determine the purity of a drug sample. Within the food industry, TLC is used for many different applications, ranging from lipid separation to food dye analysis.
Although TLC can be fully automated, it is commonly a manual technique. Consequently, aftermarket supplies and consumables dominate the market: reagents, standardized plates and capillary tubes for sample application. Altogether, such items account for majority of the total market. TLC faces competition from HPLC and, particularly in the pharmaceutical industry, flash chromatography.
The foregoing data was extracted and adapted from SDi's Global Assessment Report, 9th Edition. For more information, contact Glenn Cudiamat, VP of Research Services, Strategic Directions International, Inc., 6242 Westchester Parkway, Suite 100, Los Angeles, CA 90045, (310) 641-4982, fax: (310) 641-8851, e-mail: cudiamat@strategic-directions.com
Best of the Week: Food Analysis, Chemical Migration in Plastic Bottles, STEM Researcher of the Year
December 20th 2024Top articles published this week include the launch of our “From Lab to Table” content series, a Q&A interview about using liquid chromatography–high-resolution mass spectrometry (LC–HRMS) to assess chemical hazards in plastic bottles, and a piece recognizing Brett Paull for being named Tasmanian STEM Researcher of the Year.
Using LC-MS/MS to Measure Testosterone in Dried Blood Spots
December 19th 2024Testosterone measurements are typically performed using serum or plasma, but this presents several logistical challenges, especially for sample collection, storage, and transport. In a recently published article, Yehudah Gruenstein of the University of Miami explored key insights gained from dried blood spot assay validation for testosterone measurement.
Determination of Pharmaceuticals by Capillary HPLC-MS/MS (Dec 2024)
December 19th 2024This application note demonstrates the use of a compact portable capillary liquid chromatograph, the Axcend Focus LC, coupled to an Agilent Ultivo triple quadrupole mass spectrometer for quantitative analysis of pharmaceutical drugs in model aqueous samples.
