In-Syringe Liquid-Liquid Microextraction of Drug
Researchers from Soochow University (Taipei, Taiwan, Republic of China) applied an in-syringe liquid-liquid microextraction technique to prepare a clenbuterol sample for analysis by high performance liquid chromatography (HPLC).
Researchers from Soochow University (Taipei, Taiwan, Republic of China) applied an in-syringe liquid-liquid microextraction technique to prepare a clenbuterol sample for analysis by high performance liquid chromatography (HPLC). The researchers first drew a 10-L volume of formic acid into a 100-L syringe and then a 20-L volume of a sedimented sample containing the drug. Repeated cycling of the plunger in and out enabled extraction of the drug into the aqueous formic acid phase. They then used reversed-phase HPLC with a C18 column and an aqueous acetonitrile mobile phase to analyze the extract.
Study Explores Thin-Film Extraction of Biogenic Amines via HPLC-MS/MS
March 27th 2025Scientists from Tabriz University and the University of Tabriz explored cellulose acetate-UiO-66-COOH as an affordable coating sorbent for thin film extraction of biogenic amines from cheese and alcohol-free beverages using HPLC-MS/MS.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
