Spanish Chemists Validate KDP
KDP (long-chain dipalmitate exter of kojic acid) was recently introduced to resolve skin pigment issues resulting from age spots.
KDP (long-chain dipalmitate exter of kojic acid) was recently introduced to resolve skin pigment issues resulting from age spots. As a skin-whitening agent, KDP should be controlled, to ensure efficacy and safety. As none existed, Alberto Chisvert and colleagues from the universities of Valencia and Alicante in Spain investigated set out to use size exclusion chromatography (SEC) with UV-visible detection. Sample preparation of five samples comprised ultrasonic extraction with tetrahydrofuran (THF) and dilution to the same volume for each sample. Water was avoided because it brought about precipitation of KDP. The extract was analyzed with a SEC column of polystyrene-divinylbenzene copolymer, using THF as mobile phase. KDP levels in the commercial samples were in the range 0.34-2.14% (w/w). The researchers concluded that the speed and accuracy of the SEC method for measuring KDP in skin whiteners make it a good candidate method for quality control in the cosmetics industry.
Study Explores Thin-Film Extraction of Biogenic Amines via HPLC-MS/MS
March 27th 2025Scientists from Tabriz University and the University of Tabriz explored cellulose acetate-UiO-66-COOH as an affordable coating sorbent for thin film extraction of biogenic amines from cheese and alcohol-free beverages using HPLC-MS/MS.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
