Sample Prep Protocol Developed for Neuropeptide Research
In a recent article published in the Journal of Proteomic Research, a group of researchers reported a new two-dimensional liquid chromatography (2D LC) protocol for the preparation and analysis of rat neuropeptides.
In a recent article published in the Journal of Proteomic Research, a group of researchers reported a new two-dimensional liquid chromatography (2D LC) protocol for the preparation and analysis of rat neuropeptides.
As opposed to the more common method used to prepare samples in this area, which utilizes microwave irradiation to inactivate mammalian proteases, the protocol developed here utilized boiling water, which stops the enzymes responsible for nonspecific protein degradation. This technique produced results similar to those obtained with the microwave protocols, but was easier to implement and involved standard laboratory equipment.
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RAFA 2024 Highlights: Contemporary Food Contamination Analysis Using Chromatography
November 18th 2024A series of lectures focusing on emerging analytical techniques used to analyse food contamination took place on Wednesday 6 November 2024 at RAFA 2024 in Prague, Czech Republic. The session included new approaches for analysing per- and polyfluoroalkyl substances (PFAS), polychlorinated alkanes (PCAS), Mineral Oil Hydrocarbons (MOH), and short- and medium-chain chlorinated paraffins (SCCPs and MCCPs).
