Protein Isolation Using Multipurpose Peptide Tags
A research group at Lund University (Lund, Sweden) fused a multifunctional peptide tag to the N-terminal ends of green fluorescent protein, lactate dehydrogenase, and human hemoglobin in preparation for separation via ion-exchange chromatography, immobilized metal-ion affinity chromatography, and hydrophobic interaction chromatography.
A research group at Lund University (Lund, Sweden) fused a multifunctional peptide tag to the N-terminal ends of green fluorescent protein, lactate dehydrogenase, and human hemoglobin in preparation for separation via ion-exchange chromatography, immobilized metal-ion affinity chromatography, and hydrophobic interaction chromatography. The peptide tag consisted of histidine, tyrosine, and aspartate residues. They found the tagged green fluorescent protein to be retained longer in both hydrophobic interaction and ion-exchange chromatography.
The Chromatographic Society 2025 Martin and Jubilee Award Winners
December 6th 2024The Chromatographic Society (ChromSoc) has announced the winners of the Martin Medal and the Silver Jubilee Medal for 2025. Professor Bogusław Buszewski of Nicolaus Copernicus University in Torun, Poland, has been awarded the prestigious Martin Medal, and the 2025 Silver Jubilee Medal has been awarded to Elia Psillakis of the Technical University of Crete in Greece.
RAFA 2024 Highlights: Contemporary Food Contamination Analysis Using Chromatography
November 18th 2024A series of lectures focusing on emerging analytical techniques used to analyse food contamination took place on Wednesday 6 November 2024 at RAFA 2024 in Prague, Czech Republic. The session included new approaches for analysing per- and polyfluoroalkyl substances (PFAS), polychlorinated alkanes (PCAS), Mineral Oil Hydrocarbons (MOH), and short- and medium-chain chlorinated paraffins (SCCPs and MCCPs).
