Protein Isolation Using Multipurpose Peptide Tags
A research group at Lund University (Lund, Sweden) fused a multifunctional peptide tag to the N-terminal ends of green fluorescent protein, lactate dehydrogenase, and human hemoglobin in preparation for separation via ion-exchange chromatography, immobilized metal-ion affinity chromatography, and hydrophobic interaction chromatography.
A research group at Lund University (Lund, Sweden) fused a multifunctional peptide tag to the N-terminal ends of green fluorescent protein, lactate dehydrogenase, and human hemoglobin in preparation for separation via ion-exchange chromatography, immobilized metal-ion affinity chromatography, and hydrophobic interaction chromatography. The peptide tag consisted of histidine, tyrosine, and aspartate residues. They found the tagged green fluorescent protein to be retained longer in both hydrophobic interaction and ion-exchange chromatography.
RAFA 2024 Highlights: Contemporary Food Contamination Analysis Using Chromatography
November 18th 2024A series of lectures focusing on emerging analytical techniques used to analyse food contamination took place on Wednesday 6 November 2024 at RAFA 2024 in Prague, Czech Republic. The session included new approaches for analysing per- and polyfluoroalkyl substances (PFAS), polychlorinated alkanes (PCAS), Mineral Oil Hydrocarbons (MOH), and short- and medium-chain chlorinated paraffins (SCCPs and MCCPs).
