The Application Notebook
Dionex has developed an autosampler with very precise sample preconcentration capabilities. When analyte detection is no longer possible even with a large loop injection, sample preconcentration (loading a sample through a concentrator column and eluting the concentrate) is used.
Dionex has developed an autosampler with very precise sample preconcentration capabilities. When analyte detection is no longer possible even with a large loop injection, sample preconcentration (loading a sample through a concentrator column and eluting the concentrate) is used. Although sample preconcentration is not new, the challenge has been to match an appropriate concentrator pump with the accuracy requirement of the application and fully automate the process. As detection limits are reduced, non-reproducibility of the pumping mechanism can lead to significant error and a high relative standard deviation for the analyte of interest. The Dionex AS uniquely pairs a very highly precise syringe pump with the capabilities of an autosampler to allow sample preconcentration in an automated fashion for up to 49 samples.
The "concentrate" feature on the new AS allows the user to concentrate up to 8 mL of sample with full automation capability. The actual "concentrate" step uses the same mechanism that the autosampler uses to make traditional sample loop injections, albeit with a considerably higher volume than loop loading. With "concentrate", sample is loaded onto a concentrator column (up to 150 psi backpressure) and RSDs of below 0.5% are routinely achieved. The precision is related to the volume delivered, flow-rate across the concentrator and the capacity of the concentrator. The AS syringe pump is able to deliver precise, repeatable volumes onto the concentrator despite possible changes in backpressure. Figure 1 illustrates the use of the AS in determining low concentrations of perchlorate (5 ppb) via sample preconcentration. In this example, 5 mL of sample were injected over a concentrator column (TAC-ULP) to achieve the desired detection limits. RSDs for perchlorate at this concentration are less than 1%.
Figure 1: Using the AS to determine low concentrations of perchlorate via sample preconcentration.
With the Dionex AS, chromatographers now have automated sample preconcentration without the need to manually feed sample into a pump. This translates to direct labour savings as well as improved analysis reliability.
Investigating the Protective Effects of Frankincense Oil on Wound Healing with GC–MS
April 2nd 2025Frankincense essential oil is known for its anti-inflammatory, antioxidant, and therapeutic properties. A recent study investigated the protective effects of the oil in an excision wound model in rats, focusing on oxidative stress reduction, inflammatory cytokine modulation, and caspase-3 regulation; chemical composition of the oil was analyzed using gas chromatography–mass spectrometry (GC–MS).
Evaluating Natural Preservatives for Meat Products with Gas and Liquid Chromatography
April 1st 2025A study in Food Science & Nutrition evaluated the antioxidant and preservative effects of Epilobium angustifolium extract on beef burgers, finding that the extract influenced physicochemical properties, color stability, and lipid oxidation, with higher concentrations showing a prooxidant effect.
Rethinking Chromatography Workflows with AI and Machine Learning
April 1st 2025Interest in applying artificial intelligence (AI) and machine learning (ML) to chromatography is greater than ever. In this article, we discuss data-related barriers to accomplishing this goal and how rethinking chromatography data systems can overcome them.