Monday morning sessions
Monday Morning Sessions
HPLC 2011 kicks off to a great start with a series of keynote lectures chaired by Peter Schoenmakers. Gyula Vigh opens this session with a lecture on a new preparative-scale isoelectrophoretic trapping device and is followed by Guenther Bonn talking about the importance of novel enrichment and separation methods in bioanalysis. Massimo Morbidelli closes this session with a lecture on continuous chromatography (MCSGP) for the purification of therapeutic proteins.
Mark Schure chairs the Particle Technology session that includes a lecture from Fabrice Gritti on the challenges that need to be overcome to develop more efficient columns and technologies. The session closes with a talk by Tivadar Farkas on the performance of commercial HPLC columns made with core-shell sorbent particles.
Klára Valkó hosts the DNA and Biopharmaceuticals session that starts with a talk by Edward Yeung on the electromigration of single protein and DNA molecules in nanopores followed by a lecture on biointeraction analysis by high performance frontal affinity chromatography by Temporini Caterina. An overview of new separation media and methods for plasmid DNA analysis is presented by Michael Laemmerhofer and Frank Tomasella closes the session with a talk on quality by design in the development of an analytical chromatographic method.
The Proteomics session chaired by Robert Kennedy begins with a talk by Barry Karger on the ultra-trace proteomic analysis of 10,000 laser capture microdissection breast cancer cells using 2D LC–MS with a PLOT column followed by Alejandro Cifuentes on the future of foodomics; Sebastiaan Eeltink on the potential of polymer monolithic column technology combined with high-resolution MS to analyse protein isoforms and László Drahos on nanoUPLC–MS–MS to determine glycosylation patterns.
Poster sessions and vendor seminars take place during the lunch break.
The Complexity of Oligonucleotide Separations
January 9th 2025Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.
Metabolomics Analysis of Low Birth-Weight Infants Using UHPLC-MS/MS Following Lipid Emulsion
January 10th 2025A recent study aimed to directly compare the changes in serum metabolites among very low birth-rate (VLBW) infants following the administration of the soybean oil-based lipid emulsion and soybean oil, medium-chain triglycerides, olive oil, and fish oil (SMOF) lipid emulsion using untargeted metabolomics techniques.
Analyzing New Drug Modalities: An ISC 2024 Interview with Kelly Zhang
January 10th 2025At ISC 2024 in Liverpool, United Kingdom, LCGC International interviewed Kelly Zhang of Genentech about her work analyzing new drug modalities, such as mRNA, oligonucleotides, peptides, and cell and gene therapies.